A phylogenetic analysis of micro-organisms isolated from subsurface environments

Molecular Ecology
K P Stim

Abstract

Three methods were used to provide information on the identity and phylogenetic relatedness of 19 aerobic, chemoheterotrophic bacteria isolated from topsoil and deep subsurface sediments at a site in South Carolina. These methods were (i) analysis of selected physiological traits, (ii) restriction endonuclease analysis (REA) of genomic DNA, and (iii) analysis of 16S ribosomal RNA sequences. When the 16S rRNA sequences were compared with those for 12 standard strains, two topsoil isolates and six subsurface strains formed a tight group with the high-G+C Gram-positive bacteria and appeared to be most closely related to Arthrobacter globiformis--a coryneform-actinomycete bacterium with unusually effective survival capabilities. The rest of the subsurface isolates were scattered among the standard strains from the Proteobacteria-including the pseudomonads and Agrobacterium tumefaciens--or the low-G+C Gram-positive bacteria.

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Citations

Nov 17, 2009·Letters in Applied Microbiology·A M MazottoA B Vermelho
Oct 7, 2009·Journal of Applied Microbiology·A R CarlosR Tenreiro
May 25, 2010·FEMS Microbiology Ecology·Felipe BastidaHans H Richnow
Aug 21, 2010·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·A Al-AhmadR Kohal
Aug 18, 2005·Environmental Microbiology·Karen M KalanetraDouglas C Nelson

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