A physical-chemical and immunological comparison shows that native and renatured Escherichia coli tryptophan synthase beta 2 subunits are identical

Biochimie
A Murry-Brelier, M E Goldberg

Abstract

An acid-denaturation of the beta 2 subunit of Escherichia coli tryptophan synthase has been recently described. In the present study, renaturation yield of acid-denaturated beta 2, and the influence of temperature, protein concentration and presence of ligands are investigated. It is also demonstrated that 3 forms of the protein are obtained at the end of the renaturation process: one is fully active, and is identical to native beta 2, as indicated by some of its chemical and physical properties, as well as by its immunological reactivity towards monoclonal antibodies specific for the native protein. A second form is composed of high molecular weight insoluble and inactive aggregates. A third form consists of low molecular weight soluble and inactive aggregates. The results obtained for the immunochemical reactivity of these small aggregates indicate that they are formed with partly correctly folded beta monomers assembled by specific but incorrect quaternary interactions. The capacity of monoclonal antibodies to detect such incorrect structures and to characterize renatured proteins is particularly emphasized.

References

Sep 1, 1978·Archives of Biochemistry and Biophysics·A IkaiH Noda
Jun 1, 1975·Bacteriological Reviews·I P Crawford
Jun 4, 1975·Science·K BertrandC Yanofsky
Mar 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S Blond, M Goldberg
Jan 1, 1987·Progress in Biophysics and Molecular Biology·R Jaenicke
Jul 1, 1974·European Journal of Biochemistry·R Jaenicke
Jan 1, 1983·European Journal of Biochemistry·A N Lane, K Kirschner
Oct 5, 1980·Journal of Molecular Biology·I P CrawfordC Yanofsky
Nov 1, 1981·European Journal of Biochemistry·A N Lane, K Kirschner
Dec 21, 1981·Nucleic Acids Research·C YanofskyA M Wu
Sep 13, 2005·Oncogene·Nicolas ManelMarc Sitbon

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