A physical map and analysis of the murine C kappa-RS region show the presence of a conserved element

European Journal of Immunology
B MüllerM Reth

Abstract

Lambda-producing B lymphocytes have frequently deleted one or, more often, both Ig kappa loci. This deletion is mediated by the rearrangement of an element which lies 3' of C kappa and which is called RS (recombining sequence) in the mouse and Kde (kappa-deleting element) in the human. The tight correlation between V lambda to J lambda rearrangements and an RS-mediated deletion may indicate that sequences in the C kappa-RS region are controlling the activation of the Ig lambda locus. We have linked the C kappa exon and the RS element by phage cloning and compared the C kappa-RS region to the previously cloned human C kappa-Kde region. The distance between C kappa and RS is 25 kb and is thus similar to the distance of 24 kb separating the human C kappa exon and Kde element. Both mouse and man carry a conserved sequence of 470 bp (Rx) which lies 9 kb 3' of the mouse C kappa and 12 kb 3' of the human C kappa exon. The conserved mouse Rx sequence contains part of the kappa 3' enhancer.

References

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Citations

Jan 1, 1991·Mammalian Genome : Official Journal of the International Mammalian Genome Society·J H Nadeau, M Kosowsky
Oct 3, 1999·Cell Biochemistry and Biophysics·M W Retter, D Nemazee
Feb 22, 2012·Journal of Clinical Immunology·Wenzhao MengEline T Luning Prak
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May 29, 1998·European Journal of Immunology·T KirschbaumH G Zachau
Oct 19, 2001·Oncogene·L M Boxer, C V Dang

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