A physical sciences network characterization of circulating tumor cell aggregate transport

American Journal of Physiology. Cell Physiology
Michael R KingOwen J T McCarty

Abstract

Circulating tumor cells (CTC) have been implicated in the hematogenous spread of cancer. To investigate the fluid phase of cancer from a physical sciences perspective, the multi-institutional Physical Sciences-Oncology Center (PS-OC) Network performed multidisciplinary biophysical studies of single CTC and CTC aggregates from a patient with breast cancer. CTCs, ranging from single cells to aggregates comprised of 2-5 cells, were isolated using the high-definition CTC assay and biophysically profiled using quantitative phase microscopy. Single CTCs and aggregates were then modeled in an in vitro system comprised of multiple breast cancer cell lines and microfluidic devices used to model E-selectin mediated rolling in the vasculature. Using a numerical model coupling elastic collisions between red blood cells and CTCs, the dependence of CTC vascular margination on single CTCs and CTC aggregate morphology and stiffness was interrogated. These results provide a multifaceted characterization of single CTC and CTC aggregate dynamics in the vasculature and illustrate a framework to integrate clinical, biophysical, and mathematical approaches to enhance our understanding of the fluid phase of cancer.

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Citations

Jul 30, 2015·Blood Reviews·Annachiara MitrugnoOwen J T McCarty
Apr 20, 2016·Proceedings of the National Academy of Sciences of the United States of America·Sam H AuMehmet Toner
Oct 16, 2016·Biomechanics and Modeling in Mechanobiology·L L XiaoB M Fu
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Feb 20, 2021·PLoS Computational Biology·Sitong WangHuixin Shi
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Aug 15, 2019·ACS Applied Materials & Interfaces·Marco E MialiPaolo Decuzzi

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