A physicochemical characterization of the interaction between DC-Chol/DOPE cationic liposomes and DNA

The Journal of Physical Chemistry. B
Alberto Rodríguez-PulidoElena Junquera

Abstract

A 1:1 mixture of the cationic lipid 3beta-[ N-( N', N'-dimethylaminoethane)-carbamoyl]cholesterol hydrochloride (DC-Chol) and the zwitterionic lipid, 1,2-dioleoyl- sn-glycero-3-phosphoetanolamine (DOPE), has been used to compact calf-thymus DNA (CT-DNA) in aqueous buffered solution at 298.15 K. The formation process of this lipoplex has been analyzed by means of electrophoretic mobility, cryo-TEM, dynamic light scattering, and fluorescence spectroscopy techniques. The experimental results indicate that DC-Chol/DOPE liposomes are mostly spherical and unilamellar, with a mean diameter of around 99 +/- 10 nm and a bilayer with a thickness of 4.5 +/- 0.5 nm. In the presence of CT-DNA, DC-Chol/DOPE/CT-DNA lipoplexes are formed by means of a strong entropically driven surface electrostatic interaction, as confirmed by zeta potential and fluorescence results, as a consequence of which DNA is compacted and condensed at the surface of the cationic liposomes. The negative charges of DNA phosphate groups are neutralized by the positive charges of cationic liposomes at the isoneutrality L/ D ratio, ( L/ D) varphi around 4, obtained from electrophoretic, fluorescence, and DLS measurements. The decrease in the fluorescence emission intensity...Continue Reading

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Citations

May 25, 2010·Physical Chemistry Chemical Physics : PCCP·Manuel Alatorre-MedaJulio R Rodríguez
Sep 3, 2010·Molecular Cancer Therapeutics·Sreeram Kalarical JanardhananChuanbin Mao
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Jan 22, 2010·The Journal of Physical Chemistry. B·Yuanhua DingRong Guo
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