A physiologic flow chamber model to define intravascular ultrasound enhancement of fibrin using echogenic liposomes

Investigative Radiology
Andrew J HamiltonDavid D McPherson

Abstract

Echogenic immunoliposomes (ELIP) for enhancement of vasoactive and pathologic components of endothelium and atherosclerosis have been developed. A physiologic flow chamber model has been developed to define intravascular ultrasound enhancement of a fibrin interface. A IgG ELIP was used, which nonspecifically associated with fibrin, to demonstrate the suitability of this model. With varying doses of IgG ELIP, the fibrin wells were imaged at 1, 2, 4, 6, and 9 minutes. IgG ELIP enhanced fibrin versus saline (P < 0.005) was visible at 1 minute, lasted at least 9 minutes, and at 6 minutes the interface enhanced 27% +/- 6.1%. Enhancement was caused by increases in interface thickness and brightness. Enhancement increased with dose up to 8 mg lipid (n = 4 per time point). This model can quantitate the components of IVUS enhancement of an interface produced by ELIP. This model may allow for further development and understanding of ELIP and other targeted ultrasound contrast agents.

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Citations

Mar 8, 2005·Survey of Ophthalmology·Shehab EbrahimPaul J Lee
Mar 5, 2004·Ultrasound in Medicine & Biology·Constantin-C CoussiosDavid D McPherson
Oct 12, 2010·Stroke; a Journal of Cerebral Circulation·Kathryn E Hitchcock, Christy K Holland
Dec 19, 2009·Ultrasound in Medicine & Biology·Liselotte M KornmannArnold P G Hoeks
Nov 12, 2009·Journal of Controlled Release : Official Journal of the Controlled Release Society·Melvin E KlegermanDavid D McPherson
May 16, 2008·Advanced Drug Delivery Reviews·Shao-Ling Huang
May 26, 2004·Cardiology Clinics·Flordeliza S VillanuevaJagat Narula
Aug 8, 2009·Journal of the American College of Cardiology·Shao-Ling HuangDavid D McPherson
Mar 12, 2004·Journal of the American College of Cardiology·Andrew J HamiltonDavid D McPherson
Mar 31, 2010·Proceedings of the Institution of Mechanical Engineers. Part H, Journal of Engineering in Medicine·E P Stride, C C Coussios
Mar 22, 2018·Progress in Neuro-psychopharmacology & Biological Psychiatry·Manoj P DandekarShao-Ling Huang

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