A physiologic three-dimensional cell culture system to investigate the role of decorin in matrix organisation and cell survival

Biochemical and Biophysical Research Communications
Daniela G SeidlerElke Schönherr

Abstract

In vivo cells exist in a three-dimensional environment generated and maintained by multiple cell-cell and cell-matrix interactions. Proteoglycans, like decorin, affect these complex interactions. Thus, we sought to investigate the role of decorin in a three-dimensional environment where the matrix was generated over time by decorin-deficient fibroblasts in the presence of L-ascorbic acid 2-phosphate. The cells were viable and proliferated in response to FGF2. Decorin was incorporated in the matrix and caused a approximately 2 nm shift in the average diameter of the collagen fibrils, and the range and distribution of the fibrils became narrower and more uniform. Although there were no appreciable changes in collagen composition, we found that exogenous decorin induced the de novo synthesis of collagen I and V and cross-linked beta(I). In the early phases of the three-dimensional culture, decorin reduced apoptosis. However, following the establishment of a three-dimensional matrix, the cells did not require decorin for their survival.

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Citations

Sep 26, 2013·Diseases of Aquatic Organisms·Mona E PedersenKirsten O Hannesson
Mar 6, 2013·The Journal of Biological Chemistry·Carla BocianDaniela G Seidler
Feb 19, 2016·Journal of Anatomy·Alanna StanleyFabio Quondamatteo
Oct 13, 2012·European Journal of Cell Biology·David Denis Sofeu FeugaingManuela Viola
Apr 2, 2008·International Journal of Cosmetic Science·A PuigM Mangues
Jul 28, 2011·Journal of Cellular Physiology·Christian StockDaniela G Seidler
Nov 26, 2013·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Loren E Dupuis, Christine B Kern
Jan 23, 2014·Matrix Biology : Journal of the International Society for Matrix Biology·Katerina NikolovskaDaniela G Seidler
Feb 24, 2015·Matrix Biology : Journal of the International Society for Matrix Biology·Renato V Iozzo, Liliana Schaefer
Jan 30, 2013·Matrix Biology : Journal of the International Society for Matrix Biology·Brianne K ConnizzoLouis J Soslowsky
Jan 19, 2010·Matrix Biology : Journal of the International Society for Matrix Biology·Sebastian Kalamajski, Ake Oldberg
Sep 5, 2019·International Journal of Molecular Sciences·Justyna BuchertWiltrud Richter
Nov 2, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Daniela G SeidlerMartin Götte

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