A Phytophthora infestans RXLR effector targets plant PP1c isoforms that promote late blight disease

Nature Communications
Petra BoevinkPaul R J Birch

Abstract

Plant pathogens deliver effectors to alter host processes. Knowledge of how effectors target and manipulate host proteins is critical to understand crop disease. Here, we show that in planta expression of the RXLR effector Pi04314 enhances leaf colonization by Phytophthora infestans via activity in the host nucleus and attenuates induction of jasmonic and salicylic acid-responsive genes. Pi04314 interacts with three host protein phosphatase 1 catalytic (PP1c) isoforms, causing their re-localization from the nucleolus to the nucleoplasm. Re-localization of PP1c-1 also occurs during infection and is dependent on an R/KVxF motif in the effector. Silencing the PP1c isoforms or overexpression of a phosphatase-dead PP1c-1 mutant attenuates infection, demonstrating that host PP1c activity is required for disease. Moreover, expression of PP1c-1mut abolishes enhanced leaf colonization mediated by in planta Pi04314 expression. We argue that PP1c isoforms are susceptibility factors forming holoenzymes with Pi04314 to promote late blight disease.

References

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Citations

Apr 22, 2017·Phytopathology·Meixiang Zhang, Gitta Coaker
Apr 20, 2018·The Plant Journal : for Cell and Molecular Biology·Iga TomczynskaFelix Mauch
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Jan 5, 2021·Frontiers in Plant Science·Zunaira Afzal NaveedGul Shad Ali

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Methods Mentioned

BETA
ubiquitination
confocal microscopy
myristoylation
Y2H
co-immunoprecipitation
immunoprecipitation
transgenic
Co-IP
PCR
electrophoresis

Software Mentioned

Zen 2010
ImageJ
Adobe Illustrator
Opticon Monitor
Adobe Photoshop
Leica LCS
Elements
NIS

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