PMID: 15376541Sep 21, 2004Paper

A phytoplankton growth assay for routine in situ environmental assessments

Environmental Toxicology and Chemistry
Matilde Moreira-SantosRui Ribeiro

Abstract

This study proposes an ecologically relevant and cost-effective phytoplankton growth assay for routine in situ toxicity assessments. Assay procedures were developed applying, to the extent possible, the rationale behind the design of standard algal assays. Chlorella vulgaris was selected as test species because it grows well immobilized in alginate beads and has a wide geographic distribution. The performance of the assay in a freshwater system impacted by acid mine drainage demonstrated the suitability of assay chambers and procedures. The test system, made of inexpensive materials, allowed the rapid and easy deployment of the assay. The deployment of extra chambers at reference sites provided the ability to periodically check whether algal growth had already reached recommended growth criteria (time at which the assay should end). By deploying chambers filled with control medium at all sites, temperature was identified to explain 95% of the variation in growth. By using an artificial nutrient source shown capable of promoting algal growth according to recommended standards, toxicity from the mine effluent was distinguish from in situ nutrient limitation effects. The very good agreement (r2 = 90%) between mean in situ growth r...Continue Reading

Citations

Jul 8, 2005·Archives of Environmental Contamination and Toxicology·S SoaresR Ribeiro
May 20, 2014·Ecotoxicology·Monia RenziAlberto Basset
Feb 23, 2011·Ecotoxicology and Environmental Safety·François PerreaultWilliam Gerson Matias
Sep 14, 2010·Ecotoxicology and Environmental Safety·Isabel LopesRui Ribeiro
May 25, 2010·Environment International·Cristiano V M AraújoIgnacio Moreno-Garrido
Feb 24, 2007·The Science of the Total Environment·S C AntunesF Gonçalves
May 19, 2011·Environmental Toxicology and Chemistry·Bonny KrellRui Ribeiro
Mar 13, 2012·Environmental Toxicology and Chemistry·Rui RibeiroIsabel Lopes
Oct 12, 2010·Environmental Toxicology and Chemistry·Matilde Moreira-SantosRui Ribeiro
Oct 31, 2014·Environmental Science and Pollution Research International·Tânia VidalFernando Gonçalves
Jun 15, 2011·The Science of the Total Environment·C R MarquesF Gonçalves
May 5, 2007·Integrated Environmental Assessment and Management·Mark CraneSylvie D St-Jean
Apr 19, 2007·Environmental Science & Technology·Yarlagadda V NancharaiahVayalam P Venugopalan
Mar 4, 2020·Ecotoxicology and Environmental Safety·Ana RéNelson Abrantes

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