A piglet model for detection of hypoxic-ischemic brain injury with magnetic resonance imaging.

Acta Radiologica
B H MunkebyJ K Hald

Abstract

Early detection of hypoxic-ischemic (HI) injury in the asphyxic newborn is important because present prognostic factors are inadequate. Furthermore, therapeutic interventions may have additional benefit if initiated in time. To assess whether the use of a combined protocol including conventional magnetic resonance imaging (MRI), diffusion-weighted imaging (DWI), diffusion tensor imaging (DTI), and proton MR spectroscopy (MRS) could detect pathological findings in a piglet model 7 hours after HI. Ten piglets were submitted to HI for 30 min followed by reoxygenation with 21% O2 for 7 hours. MRI at 1.5T was done prior to and 7 hours after the HI. Single-voxel proton MRS was performed, and apparent diffusion coefficient (ADC) and fractional anisotropy (FA) were measured in the basal ganglia. MRS identified N-acetylaspartate (NAA), choline (Cho), creatine (Cr), and lactate (Lac). Histology and microtubule-associated protein 2 (MAP-2) staining was performed in the basal ganglia at the end of the experiment. Compared to baseline, ADC, NAA/Cho, and NAA/Cr were significantly reduced after 7 hours (P<0.001, P=0.01, and P=0.05, respectively) and FA values were increased (P<0.025). The ratios of Lac/Cho and Lac/NAA were significantly highe...Continue Reading

Citations

Jul 11, 2012·Developmental Neuroscience·Matthew S ConradRodney W Johnson
Apr 20, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Alexander DrobyshevskySidhartha Tan
Nov 12, 2014·Annual Review of Animal Biosciences·Matthew S Conrad, Rodney W Johnson
Jul 28, 2016·Frontiers in Neuroscience·Hector LafuenteJose A Martinez-Orgado
Dec 16, 2017·Interactive Cardiovascular and Thoracic Surgery·Ren WangFeng Zhu
Jan 20, 2012·Pediatric Research·Matthew S ConradRodney W Johnson
Feb 3, 2012·European Journal of Nuclear Medicine and Molecular Imaging·Charlotte de LangeBerit H Munkeby
Jan 17, 2012·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Shai ShrotYoram Cohen
Nov 2, 2016·Pediatric Research·Håvard T GarbergOla Didrik Saugstad

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