A pilot study on post-thawing quality of Iberian red deer spermatozoa (epididymal and electroejaculated) depending on glycerol concentration and extender osmolality

Theriogenology
Felipe Martínez-PastorLuis Anel

Abstract

The optimization of cryopreservation extenders is a fundamental issue for adequately performing germplasm banking on wild species. We have tested two glycerol concentrations (4 and 8%), and three extender osmolalities (320, 380 and 430 mOsm/kg; before adding cryoprotectants), for cryopreservation of epididymal and ejaculated sperm samples from Iberian red deer. All the extenders were based on Tes-Tris and fructose (for osmolality adjustment), and complemented with 20% egg yolk. Epididymal and ejaculated sperm samples were obtained from the cauda epididymis (post-mortem) and using electroejaculation, respectively. Samples were diluted 1:1 with each extender and equilibrated for 2 h at 5 degrees C. Then, they were diluted down to 100x10(6) sperm/mL and frozen at -20 degrees C/min. Post-thawed samples were assessed for motility (CASA), HOS test, proportion of swollen (osmotically challenged) cells in the untreated sample, viability and acrosomal status. For epididymal samples, 8% glycerol rendered a slightly higher proportion of intact acrosomes on viable spermatozoa than 4%; regarding extender osmolality, 380 and 430 mOsm/kg rendered higher motility results, and the 430 mOsm/kg yielded the lowest proportion of swollen spermatozoa...Continue Reading

References

Jun 14, 2000·Animal Reproduction Science·M Yoshida
Aug 5, 2000·Animal Reproduction Science·S Salamon, W M Maxwell
Aug 5, 2000·Animal Reproduction Science·G W AsherG Evans
Apr 10, 2002·Seminars in Reproductive Medicine·Yuksel Agca, John K Critser
May 24, 2003·Theriogenology·Ana Josefa SolerJosé Julián Garde
Aug 11, 2004·Animal Reproduction Science·Myrthe T Wessel, Barry A Ball
Sep 24, 2004·Biology of Reproduction·Felipe Martinez-PastorPaulino de Paz

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Citations

Oct 2, 2012·Animal Reproduction Science·Luis Anel-LópezFelipe Martínez-Pastor
Dec 6, 2011·Animal Reproduction Science·J Tamayo-CanulF Martínez-Pastor
Sep 8, 2009·Theriogenology·A E Domínguez-RebolledoF Martínez-Pastor
Feb 2, 2010·Reproduction in Domestic Animals = Zuchthygiene·Á E Domínguez-RebolledoJ J Garde
Sep 23, 2008·Reproduction in Domestic Animals = Zuchthygiene·L AnelP de Paz
Jan 4, 2013·Reproduction in Domestic Animals = Zuchthygiene·L O TeodoroF F Souza
Aug 4, 2010·Reproduction in Domestic Animals = Zuchthygiene·A E Domínguez-RebolledoM R Fernández-Santos
Sep 1, 2015·Animal Reproduction Science·Patrícia M PapaInes Cristina Giometti
Nov 26, 2018·Reproduction in Domestic Animals = Zuchthygiene·Davide MonacoNikorn Thongtip
Jan 1, 2013·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Samantha M FavorettoJosé M B Duarte
Nov 18, 2017·Environmental Science and Pollution Research International·Graciela Quintana SaalfeldElton Pinto Colares
Jul 2, 2020·Biopreservation and Biobanking·Maria C L AlvarezJosé M B Duarte
Sep 23, 2020·Microscopy Research and Technique·Duanny Murinelly de Souza CunhaDárcio Ítalo Alves Teixeira
May 11, 2010·Reproduction, Fertility, and Development·Alvaro E Domínguez-RebolledoJ Julián Garde

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