A Plant Biologist's Toolbox to Study Translation

Frontiers in Plant Science
Serina M Mazzoni-Putman, Anna N Stepanova

Abstract

Across a broad range of species and biological questions, more and more studies are incorporating translation data to better assess how gene regulation occurs at the level of protein synthesis. The inclusion of translation data improves upon, and has been shown to be more accurate than, transcriptional studies alone. However, there are many different techniques available to measure translation and it can be difficult, especially for young or aspiring scientists, to determine which methods are best applied in specific situations. We have assembled this review in order to enhance the understanding and promote the utilization of translational methods in plant biology. We cover a broad range of methods to measure changes in global translation (e.g., radiolabeling, polysome profiling, or puromycylation), translation of single genes (e.g., fluorescent reporter constructs, toeprinting, or ribosome density mapping), sequencing-based methods to uncover the entire translatome (e.g., Ribo-seq or translating ribosome affinity purification), and mass spectrometry-based methods to identify changes in the proteome (e.g., stable isotope labeling by amino acids in cell culture or bioorthogonal noncanonical amino acid tagging). The benefits and ...Continue Reading

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Citations

May 28, 2020·Journal of Experimental Botany·Udaykumar KageKemal Kazan
Jan 11, 2019·International Journal of Molecular Sciences·Jing ZhaoGong Zhang
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Oct 11, 2021·Plant Methods·Alexander A TyurinIrina V Goldenkova-Pavlova

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Methods Mentioned

BETA
transgenics
electrophoresis
transgenic
RNA-seq
salt stress
affinity purification
Fluorescence Activated Cell Sorting
Ribo-seq
TCP-seq
chip

Software Mentioned

TRAP
5PSeq
TCP
seq
FUNCAT
SILIP
SUnSET
PSeq
Ribo
HILEP

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