A plant Bro1 domain protein BRAF regulates multivesicular body biogenesis and membrane protein homeostasis

Nature Communications
Jinbo ShenLiwen Jiang

Abstract

Plant development, defense, and many physiological processes rely on the endosomal sorting complex required for transport (ESCRT) machinery to control the homeostasis of membrane proteins by selective vacuolar degradation. Although ESCRT core components are conserved among higher eukaryotes, the regulators that control the function of the ESCRT machinery remain elusive. We recently identified a plant-specific ESCRT component, FREE1, that is essential for multivesicular body/prevacuolar compartment (MVB/PVC) biogenesis and vacuolar sorting of membrane proteins. Here we identify a plant-specific Bro1-domain protein BRAF, which regulates FREE1 recruitment to the MVB/PVC membrane by competitively binding to the ESCRT-I component Vps23. Altogether, we have successfully identified a role for BRAF, whose function as a unique evolutionary ESCRT regulator in orchestrating intraluminal vesicle formation in MVB/PVCs and the sorting of membrane proteins for degradation in plants makes it an important regulatory mechanism underlying the ESCRT machinery in higher eukaryotes.

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Citations

Jun 24, 2019·The New Phytologist·Liang ZhangJinxing Lin
Aug 17, 2019·Journal of Cell Science·Niccolò MosessoErika Isono
Aug 14, 2020·Journal of Integrative Plant Biology·Zhidan XiaoWenjin Shen
Jun 13, 2020·Frontiers in Plant Science·Maximilian Schwihla, Barbara Korbei
Nov 11, 2019·Science China. Life Sciences·Dongmei ZhuJinbo Shen
Jan 9, 2019·Frontiers in Plant Science·Yong CuiLiwen Jiang
Oct 16, 2020·The Plant Journal : for Cell and Molecular Biology·Jianyang LiuYoufa Cheng
May 15, 2020·Trends in Plant Science·Yong CuiLiwen Jiang
May 4, 2021·Frontiers in Plant Science·Mengdi ZhangJinbo Shen

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Datasets Mentioned

BETA
UBQ10

Methods Mentioned

BETA
transgenic
reverse transcription-PCR
FRET
two-hybrid
Y2H
immunoprecipitation
pull-down
pull down
PCR
electrophoresis

Software Mentioned

ClustalX2
SAMTOOLS
Lipid
ImageJ
GPS
MEGA7
ADOBE PHOTOSHOP
BLASTP
SHORE

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