A plant virus movement protein forms ringlike complexes with the major nucleolar protein, fibrillarin, in vitro

Journal of Molecular Biology
Elisabetta CanettaMichael E Taliansky

Abstract

Fibrillarin, one of the major proteins of the nucleolus, has methyltransferase activity directing 2'-O-ribose methylation of rRNA and snRNAs and is required for rRNA processing. The ability of the plant umbravirus, groundnut rosette virus, to move long distances through the phloem, the specialized plant vascular system, has been shown to strictly depend on the interaction of one of its proteins, the ORF3 protein (protein encoded by open reading frame 3), with fibrillarin. This interaction is essential for several stages in the groundnut rosette virus life cycle such as nucleolar import of the ORF3 protein via Cajal bodies, relocalization of some fibrillarin from the nucleolus to cytoplasm, and assembly of cytoplasmic umbraviral ribonucleoprotein particles that are themselves required for the long-distance spread of the virus and systemic infection. Here, using atomic force microscopy, we determine the architecture of these complexes as single-layered ringlike structures with a diameter of 18-22 nm and a height of 2.0+/-0.4 nm, which consist of several (n=6-8) distinct protein granules. We also estimate the molar ratio of fibrillarin to ORF3 protein in the complexes as approximately 1:1. Based on these data, we propose a model o...Continue Reading

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Citations

Jul 26, 2011·Nucleic Acids Research·D V RakitinaN O Kalinina
Nov 16, 2011·Plant Physiology·Peter Shaw, John Brown
Dec 17, 2008·EMBO Reports·Lesley A Stark, Michael Taliansky
Dec 3, 2010·The FEBS Journal·Soumya Roy Chowdhury, Handanahal Subbarao Savithri
Jan 1, 2014·Biochimica Et Biophysica Acta·Anna Salvetti, Anna Greco
Jul 29, 2010·The Biochemical Journal·Jens Tilsner, Karl J Oparka
Oct 12, 2016·RNA Biology·Andrew J LoveMichael E Taliansky
Sep 4, 2019·Cell Biochemistry and Function·Javier Adrian MoralesEnrique Castano
Aug 16, 2019·Molecular Plant-microbe Interactions : MPMI·Min XuRichard Kormelink
May 27, 2020·Annual Review of Virology·Madhu KappagantuJames N Culver
Feb 27, 2018·Frontiers in Plant Science·Natalia O KalininaMichael Taliansky
Feb 25, 2018·Current Opinion in Plant Biology·Svetlana Y Folimonova, Jens Tilsner

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Methods Mentioned

BETA
atomic force microscopy
AFM

Software Mentioned

JPK
Adobe Photoshop
ImageJ

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