A plaque assay for all cells secreting Ig of a given type or class

European Journal of Immunology
E GronowiczF Melchers

Abstract

A modification of the hemolytic plaque assay using protein A-coated red cells is described which makes use of the fact that the Fc portion of IgG binds to protein A. A number of murine plasmacytomas secreting different classes of Ig have been tested for plaque formation with these indicator red cells. In the presence of complement-binding antibodies specific for the corresponding class of secreted Ig, between 10 and 70% of all plated myeloma cells formed plaques. The assay shows a prozone effect in excess of antibody, suggesting that complexes of antibody and secreted Ig effect lysis of the target cells. This assay can be used to enumerate cells secreting any molecules for which complement-binding antibodies are available.

References

Jan 1, 1975·Scandinavian Journal of Immunology·F MelchersJ Andersson
Dec 1, 1972·European Journal of Immunology·W W Bullock, E Möller
Apr 5, 1974·Nature·G A Molinaro, S Dray
Apr 26, 1963·Science·N K Jerne, A A Nordin

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Citations

Jun 30, 1998·European Journal of Immunology·L CederbomF Ivars
Aug 1, 1988·Archives of Toxicology·U HurtenbachE Gleichmann
Jul 1, 1990·Transplant International : Official Journal of the European Society for Organ Transplantation·S TiisalaP Häyry
Jan 1, 1982·Immunogenetics·L L LanierG Haughton
Jul 1, 1982·Journal of Clinical Immunology·D S BankerS G Pahwa
Apr 1, 1983·Journal of Clinical Immunology·Z BrahmiR L Baehner
Apr 1, 1982·Journal of Clinical Immunology·T SakaneT Tsunematsu
Jan 1, 1981·Springer Seminars in Immunopathology·D Y Leung, R S Geha
Jan 1, 1997·Pathology Oncology Research : POR·Rudolf MihalikLászl&oacute Kopper
Jan 1, 1980·Journal of Immunological Methods·J Kettman, M Wetzel
Jan 1, 1981·Journal of Immunological Methods·G F Burns, B L Pike
Jan 1, 1981·Journal of Immunological Methods·G T PangR J Booth
Jul 30, 1982·Journal of Immunological Methods·Y TannoT Takishima
Nov 26, 1982·Journal of Immunological Methods·M A BaigH V Malling
Sep 30, 1982·Journal of Immunological Methods·T EgelandO J Mellbye
Jan 1, 1982·Journal of Immunological Methods·C L Librach, I R Mackay
Apr 29, 1983·Journal of Immunological Methods·P Tauris, F Jørgensen
Sep 16, 1983·Journal of Immunological Methods·S Makonkawkeyoon, V Vithayasai
Jul 29, 1983·Journal of Immunological Methods·G H BoerrigterR J Scheper
May 27, 1983·Journal of Immunological Methods·R AggerV Andersen
Aug 2, 1985·Journal of Immunological Methods·L MayerH G Kunkel
Jan 22, 1986·Journal of Immunological Methods·Y YamadaR Green
May 22, 1986·Journal of Immunological Methods·D Levitt, R Danen
Aug 3, 1987·Journal of Immunological Methods·D D WuR A Phillips
Jul 22, 1988·Journal of Immunological Methods·A J UlmerH D Flad

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