PMID: 2105303Feb 1, 1990Paper

A plasmid vector with a selectable marker for halophilic archaebacteria

Journal of Bacteriology
M L Holmes, M L Dyall-Smith

Abstract

A mutant resistant to the gyrase inhibitor novobiocin was selected from a halophilic archaebacterium belonging to the genus Haloferax. Chromosomal DNA from this mutant was able to transform wild-type cells to novobiocin resistance, and these transformants formed visible colonies in 3 to 4 days on selective plates. The resistance gene was isolated on a 6.7-kilobase DNA KpnI fragment, which was inserted into a cryptic multicopy plasmid (pHK2) derived from the same host strain. The recombinant plasmid transformed wild-type cells at a high efficiency (greater than 10(6)/micrograms), was stably maintained, and could readily be reisolated from transformants. It could also transform Halobacterium volcanii and appears to be a useful system for genetic analysis in halophilic archaebacteria.

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Citations

Apr 12, 2000·Molecular Microbiology·M L Holmes, M L Dyall-Smith
Jun 28, 2011·PloS One·Mike L Dyall-SmithDieter Oesterhelt
Apr 16, 2013·Archaea : an International Microbiological Journal·Kate PorterMike Dyall-Smith
May 19, 2016·PloS One·Yunjun MeiXiangdong Chen
Mar 30, 2017·Archaea : an International Microbiological Journal·Yuchen WangXiangdong Chen
Jul 1, 1995·Bio/technology·M W AdamsR M Kelly
May 31, 2007·Current Microbiology·Yunjun MeiPing Shen
Jan 16, 2009·Biochemical Society Transactions·Michaela WagnerSonja-Verena Albers
Jan 1, 1995·World Journal of Microbiology & Biotechnology·M CiaramellaM Rossi
Feb 20, 2015·Microbiology and Molecular Biology Reviews : MMBR·Haina WangQunxin She
Sep 15, 2010·Applied Microbiology and Biotechnology·Hiroko TokunagaMasao Tokunaga
Dec 3, 2015·Environmental Microbiology·Salla T JaakkolaDennis H Bamford
Jan 5, 2005·Nature Reviews. Genetics·Thorsten Allers, Moshe Mevarech
May 11, 2002·Journal of Bacteriology·Christian Wanner, Jörg Soppa
Nov 21, 2007·Canadian Journal of Microbiology·Yunjun MeiXiangdong Chen
Jan 22, 2010·Journal of Virology·Elina RoineDennis H Bamford
Dec 2, 2020·Open Biology·Patricia Pérez-ArnaizThorsten Allers
Jun 11, 1998·Journal of Bacteriology·R CannioS Bartolucci
Oct 10, 1998·Journal of Virology·C Bath, M L Dyall-Smith
Feb 3, 1995·Gene·M L HolmesM L Dyall-Smith
Jun 4, 1999·Trends in Microbiology·K R Sowers, H J Schreier
Apr 1, 1995·Journal of Virology·S D Nuttall, M L Dyall-Smith
Feb 1, 1993·Journal of Bacteriology·A S AkhmanovaA S Mankin
Sep 1, 1995·Applied and Environmental Microbiology·M YasudaT Oshima
Jun 25, 2015·Microbiology Spectrum·Patrick ForterreNicolas Soler
Dec 1, 1990·Journal of Bacteriology·D T Nieuwlandt, C J Daniels
Jan 1, 1991·Journal of Bacteriology·M L Holmes, M L Dyall-Smith

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