A Plasmodium phospholipase is involved in disruption of the liver stage parasitophorous vacuole membrane

PLoS Pathogens
Paul-Christian BurdaVolker T Heussler

Abstract

The coordinated exit of intracellular pathogens from host cells is a process critical to the success and spread of an infection. While phospholipases have been shown to play important roles in bacteria host cell egress and virulence, their role in the release of intracellular eukaryotic parasites is largely unknown. We examined a malaria parasite protein with phospholipase activity and found it to be involved in hepatocyte egress. In hepatocytes, Plasmodium parasites are surrounded by a parasitophorous vacuole membrane (PVM), which must be disrupted before parasites are released into the blood. However, on a molecular basis, little is known about how the PVM is ruptured. We show that Plasmodium berghei phospholipase, PbPL, localizes to the PVM in infected hepatocytes. We provide evidence that parasites lacking PbPL undergo completely normal liver stage development until merozoites are produced but have a defect in egress from host hepatocytes. To investigate this further, we established a live-cell imaging-based assay, which enabled us to study the temporal dynamics of PVM rupture on a quantitative basis. Using this assay we could show that PbPL-deficient parasites exhibit impaired PVM rupture, resulting in delayed parasite egr...Continue Reading

References

Aug 15, 1994·Annals of the New York Academy of Sciences·D A Portnoy, S Jones
Feb 20, 1999·Protein Expression and Purification·P SheffieldZ Derewenda
May 8, 1999·Acta Crystallographica. Section D, Biological Crystallography·P J SheffieldZ S Derewenda
Feb 19, 2000·Current Opinion in Microbiology·W Goebel, M Kuhn
Dec 2, 2000·Biochimie·R Rosenstein, F Götz
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Nov 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Kimie KadotaMasao Yuda
Dec 14, 2004·The Journal of Biological Chemistry·Purnima BhanotVictor Nussenzweig
Jan 17, 2008·Nature Reviews. Microbiology·Kevin Hybiske, Richard S Stephens
Apr 19, 2008·Cellular Microbiology·Anja Schmidt-ChristensenVolker T Heussler
May 15, 2009·Cellular Microbiology·Tomoko IshinoPatricia Baldacci
Nov 27, 2009·The Journal of Biological Chemistry·Adebola FalaePurnima Bhanot
Dec 31, 2009·Nucleic Acids Research·Ronald GodiskaNikolai Ravin
Jun 10, 2011·Experimental Parasitology·Andreas RaabeKai Wengelnik
Oct 25, 2011·Nature Methods·Claudia PfanderOliver Billker
Aug 18, 2012·International Journal of Medical Microbiology : IJMM·Tobias SpielmannKai Matuschewski
Dec 4, 2012·Microbiology and Molecular Biology Reviews : MMBR·Nikolas FriedrichThierry Soldati
Feb 8, 2013·Autophagy·Nina EickelVolker T Heussler
Jun 4, 2013·Current Opinion in Microbiology·Michael J Blackman, Vern B Carruthers
Oct 4, 2013·The Journal of Biological Chemistry·Lina TawkJean-Christophe Barale

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Citations

Jun 1, 2016·Scientific Reports·Hirdesh KumarFriedrich Frischknecht
Mar 1, 2017·Cold Spring Harbor Perspectives in Medicine·Ashley M Vaughan, Stefan H I Kappe
Feb 7, 2018·MSphere·Cilly Bernardette SchniderPaul-Christian Burda
Apr 13, 2017·MBio·Paul-Christian BurdaVolker T Heussler
Dec 24, 2015·The Journal of Biological Chemistry·Viviana PszennyIsabelle Coppens
May 31, 2019·Life Science Alliance·Hadi Hasan ChoudharySatish Mishra
Sep 22, 2020·PLoS Pathogens·Elyzana Dewi PutriantiAlyssa Ingmundson
Sep 26, 2019·Malaria Journal·Reto CaldelariVolker Heussler
Aug 31, 2017·Scientific Reports·Paul-Christian BurdaVolker T Heussler
Dec 1, 2017·PLoS Medicine·UNKNOWN malERA Refresh Consultative Panel on Basic Science and Enabling Technologies
Mar 7, 2018·MSphere·Aric J Schultz, Vern B Carruthers
Sep 3, 2016·FEMS Microbiology Reviews·Emma S Sherling, Christiaan van Ooij
Aug 16, 2019·Proceedings of the National Academy of Sciences of the United States of America·Pallavi SinghChetan E Chitnis
May 7, 2019·Briefings in Functional Genomics·José L Ruiz, Elena Gómez-Díaz
Jan 26, 2020·Nature Reviews. Microbiology·Joachim M MatzMichael J Blackman
Jun 25, 2020·Scientific Reports·Suthee MangmeeOnrapak Reamtong
May 29, 2018·Science Advances·Mariana De NizMatthias Marti
Mar 10, 2021·Journal of Cell Science·Michele S Y Tan, Michael J Blackman
Mar 19, 2021·Frontiers in Cellular and Infection Microbiology·Kathrin WitmerMara K N Lawniczak
May 21, 2021·Journal of Cell Science·Mariana De NizCarolina Agop-Nersesian

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Methods Mentioned

BETA
PCR
transfection
FACS
FCS
transgenic
genetic
light microscopy

Software Mentioned

ImageJ
GraphPad
GraphPad Prism

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