A Plasmodium yoelii Mei2-Like RNA Binding Protein Is Essential for Completion of Liver Stage Schizogony

Infection and Immunity
Dorender A DankwaAshley M Vaughan

Abstract

Plasmodium parasites employ posttranscriptional regulatory mechanisms as their life cycle transitions between host cell invasion and replication within both the mosquito vector and mammalian host. RNA binding proteins (RBPs) provide one mechanism for modulation of RNA function. To explore the role of Plasmodium RBPs during parasite replication, we searched for RBPs that might play a role during liver stage development, the parasite stage that exhibits the most extensive growth and replication. We identified a parasite ortholog of the Mei2 (Meiosis inhibited 2) RBP that is conserved among Plasmodium species (PlasMei2) and exclusively transcribed in liver stage parasites. Epitope-tagged Plasmodium yoelii PlasMei2 was expressed only during liver stage schizogony and showed an apparent granular cytoplasmic location. Knockout of PlasMei2 (plasmei2(-)) in P. yoelii only affected late liver stage development. The P. yoelii plasmei2(-) liver stage size increased progressively until late in development, similar to wild-type parasite development. However, P. yoelii plasmei2(-) liver stage schizonts exhibited an abnormal DNA segregation phenotype and failed to form exoerythrocytic merozoites. Consequently the cellular integrity of P. yoel...Continue Reading

References

Nov 1, 1990·Trends in Genetics : TIG·R EgelD Weilguny
Aug 26, 1998·Cellular and Molecular Life Sciences : CMLS·T J GuilfoyleG Hagen
Feb 27, 2004·Development Genes and Evolution·Daniel C JeffaresBruce Veit
Nov 3, 2004·Genome Research·Karine G Le RochElizabeth A Winzeler
Aug 5, 2006·Science·Gunnar R MairAndrew P Waters
Aug 4, 2007·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Yuriko Harigaya, Masayuki Yamamoto
Jan 4, 2008·Proceedings of the National Academy of Sciences of the United States of America·Alice S TarunStefan H I Kappe
Dec 11, 2008·Cellular Microbiology·Ashley M VaughanStefan H I Kappe
Oct 9, 2009·The Journal of Infectious Diseases·Agnes MwakingweKami Kim
Nov 20, 2009·PloS One·Ivo H J PloemenBlandine M D Franke-Fayard
Dec 23, 2009·Molecular Microbiology·Masao YudaIzumi Kaneko
Nov 16, 2010·Current Opinion in Plant Biology·Jun Xu, Nam-Hai Chua
Dec 4, 2010·Molecular and Biochemical Parasitology·Heather J PainterManuel Llinás
Aug 2, 2011·Cellular Microbiology·Rebecca R StanwayVolker T Heussler
Nov 19, 2011·FEMS Microbiology Reviews·Stefanie GraeweVolker T Heussler
Dec 15, 2011·Nucleic Acids Research·Arnaud ChêneArtur Scherf
Mar 13, 2012·Trends in Immunology·Noah S ButlerStefan H I Kappe
May 9, 2012·Current Opinion in Biotechnology·Shahid M KhanSebastian A Mikolajczak
Jan 18, 2013·Molecular & Cellular Proteomics : MCP·Scott E LindnerStefan H I Kappe
Nov 4, 2015·BMC Genomics·B P Niranjan ReddyScott E Lindner

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Citations

Oct 30, 2016·Trends in Parasitology·Mirko Singer, Friedrich Frischknecht
Mar 1, 2017·Cold Spring Harbor Perspectives in Medicine·Ashley M Vaughan, Stefan H I Kappe
Dec 28, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Debashree GoswamiStefan H I Kappe
Jun 3, 2020·JCI Insight·Debashree GoswamiStefan Hi Kappe
Jun 18, 2017·Frontiers in Cellular and Infection Microbiology·Oriana KreutzfeldKai Matuschewski

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