PMID: 11607713Oct 15, 1996Paper

A plastid enzyme arrested in the step of precursor translocation in vivo

Proceedings of the National Academy of Sciences of the United States of America
Steffen ReinbotheK. Apel

Abstract

The key enzyme of chlorophyll biosynthesis in higher plants, NADPH:protochlorophyllide (Pchlide) oxidoreductase (POR, EC 1.3.1.33), accumulates in its precursor form (pPORA) in barley. pPORA is bound to the chloroplasts and is able to interact with the enzyme's substrate, Pchlide, at both the cytosolic as well as the stromal side of the plastid envelope. The interaction with intraplastidic Pchlide, formed in ATP-containing chloroplasts upon feeding with -aminolevulinic acid, drives vectorial translocation of pPORA across the plastid envelope membranes. In contrast, exogenously applied Pchlide causes the release of the envelope-bound precursor protein to the cytosol. Both processes compete with each other if intra- and extraplastidic Pchlide are applied simultaneously. A cytosolic heat shock cognate protein of Mr 70,000 present in wheat germ and barley leaf protein extracts appears to prevent the release of the pPORA to the cytosol in vivo, however.

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Citations

Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·S ReinbotheC Reinbothe
May 8, 2007·Proceedings of the National Academy of Sciences of the United States of America·Andreas SchemenewitzSteffen Reinbothe
Aug 5, 1997·Proceedings of the National Academy of Sciences of the United States of America·C ReinbotheS Reinbothe
Feb 11, 2004·Proceedings of the National Academy of Sciences of the United States of America·Steffen ReinbotheChristiane Reinbothe
Mar 19, 2005·The Plant Journal : for Cell and Molecular Biology·Steffen ReinbotheChristiane Reinbothe
Apr 19, 2003·Physiologia Plantarum·Henrik AronssonClas Dahlin
Jun 4, 2019·Frontiers in Plant Science·Steffen ReinbotheJohn Gray
Oct 18, 2020·Biological Chemistry·Daniel WittmannBernhard Grimm
Sep 19, 2003·Biochemical and Biophysical Research Communications·Ario de MarcoRaymonde Fonné-Pfister
Mar 30, 2004·Plant Physiology·Christiane ReinbotheSteffen Reinbothe

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