Oct 27, 2017

A platform for efficient identification of molecular phenotypes of brain-wide neural circuits

Scientific Reports
Tao JiangJing Yuan

Abstract

A neural circuit is a structural-functional unit of achieving particular information transmission and processing, and have various inputs, outputs and molecular phenotypes. Systematic acquisition and comparative analysis of the molecular features of neural circuits are crucial to elucidating the operating mechanisms of brain function. However, no efficient, systematic approach is available for describing the molecular phenotypes of specific neural circuits at the whole brain scale. In this study, we developed a rapid whole-brain optical tomography method and devised an efficient approach to map brain-wide structural and molecular information in the same brain: rapidly imaging and sectioning the whole brain as well as automatically collecting all slices; conveniently selecting slices of interest through quick data browsing and then performing post hoc immunostaining of selected slices. Using this platform, we mapped the brain-wide distribution of inputs to motor, sensory and visual cortices and determined their molecular phenotypes in several subcortical regions. Our platform significantly enhances the efficiency of molecular phenotyping of neural circuits and provides access to automation and industrialization of cell type anal...Continue Reading

  • References39
  • Citations6

References

  • References39
  • Citations6

Mentioned in this Paper

Molecular Imaging
Study
Classification
Sectioning Technique
Brain
Neural Stem Cells
Brain Function
Subcortical
Disease Transmission
Tomography, Optical

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