PMID: 1196300Jan 1, 1975Paper

A pleiotropic regulatory mutation in lambda bacteriophage

Molecular & General Genetics : MGG
A HonigmanA B Oppenheim

Abstract

Lambda bacteriophage mutants, lambdasar, were isolated. These mutants can form plaques on a non lysogenic lawn and are unable to grow on nonimmune (imm-), cro constitutive hosts. Analysis of the restriction of lambdasar by a set of defective lysogens suggested that both the cro and cII gene products participate in the inhibition. The sar mutations were mapped in the ori region between the genes cII and O. Complementation experiments showed that under the restrictive conditions lamdasar is defective in the expression of both the N and the O genes. Transcription analyses support these findings, as lambdasar is unable to serve as a template for transcription after infecting cro constitutive hosts. In addition lambdasar does not replicate under the restrictive conditions, although its DNA can bind to the host membrane to some extent. The Sar phenotype can be relieved by removing sites of action of cro either by a V2 mutation or by substituting the lambda immunity region by imm434 or imm21. Similarly introducing a cy mutation, which interferes with the action of the cII gene product, also eliminates the Sar effect. The sar mutation can suppress cy mutations as manifested in plaque morphology, lysogenization frequency, cI repressor s...Continue Reading

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Citations

Mar 22, 1976·Molecular & General Genetics : MGG·S Tokuno, M Gough
Dec 1, 1976·Virology·A Oppenheim, A B Oppenheim
Jun 15, 1977·Virology·A OppenheimA B Oppenheim
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Dec 1, 1978·Journal of Virology·C L TruittJ R Walker

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