A 'Plug and Play' Platform for the Production of Diverse Monoterpene Hydrocarbon Scaffolds in Escherichia coli

ChemistrySelect
Nicole G H LeferinkNigel S Scrutton

Abstract

The terpenoids constitute one of the largest and most diverse classes of natural compounds with applications as pharmaceuticals, flavorings and fragrances, pesticides and biofuels. Synthetic biology is ideally placed to create new routes to this chemical diversity and facilitation of new compound discovery. The C10 monoterpenoids display a huge structural diversity produced from a single substrate, geranyl diphosphate, by a family of monoterpene cyclases and synthases (mTC/S). Here we employ a library of mTC/S in a single 'plug and play' platform system for the production of over 30 different monoterpenoids in Escherichia coli by fermentation on glucose. These products include several compounds never before produced in engineered microbes demonstrating the power of this approach to rapidly create routes to structural diversity.

References

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Citations

Aug 17, 2019·Scientific Reports·Nicole G H LeferinkNigel S Scrutton
Nov 5, 2019·Chembiochem : a European Journal of Chemical Biology·Nicole G H LeferinkNigel S Scrutton
May 1, 2020·PloS One·Gajendar Komati ReddyEriko Takano
Mar 16, 2019·Synthetic Biology·Quentin M DudleyMichael C Jewett
Sep 28, 2018·Scientific Reports·Aitor Hernandez-OrtegaNigel S Scrutton
Nov 16, 2018·Chembiochem : a European Journal of Chemical Biology·Gabriel A Ascue AvalosNigel S Scrutton
Feb 19, 2019·Technological Forecasting and Social Change·Barbara Ribeiro, Philip Shapira
May 4, 2021·Chembiochem : a European Journal of Chemical Biology·Clara A FerrazNigel S Scrutton
Jun 3, 2021·Microorganisms·Alec BannerNigel S Scrutton
Jul 8, 2021·Natural Product Reports·Mauro A RinaldiNigel S Scrutton
Mar 26, 2019·Journal of the American Chemical Society·Efrat PahimaDan T Major
Oct 21, 2021·Chembiochem : a European Journal of Chemical Biology·Nicole G H Leferink, Nigel S Scrutton
Jan 6, 2022·ACS Synthetic Biology·Simon d'OelsnitzAndrew D Ellington

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Methods Mentioned

BETA
PCR

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