A "plug-n-play" modular metabolic system for the production of apocarotenoids

Biotechnology and Bioengineering
Congqiang ZhangHeng-Phon Too

Abstract

Apocarotenoids, such as α-, β-ionone, and retinol, have high commercial values in the food and cosmetic industries. The demand for natural ingredients has been increasing dramatically in recent years. However, attempts to overproduce β-ionone in microorganisms have been limited by the complexity of the biosynthetic pathway. Here, an Escherichia coli-based modular system was developed to produce various apocarotenoids. Incorporation of enzyme engineering approaches (N-terminal truncation and protein fusion) into modular metabolic engineering strategy significantly improved α-ionone production from 0.5 mg/L to 30 mg/L in flasks, producing 480 mg/L of α-ionone in fed-batch fermentation. By modifying apocarotenoid genetic module, this platform strain was successfully re-engineered to produce 32 mg/L and 500 mg/L of β-ionone in flask and bioreactor, respectively (>80-fold higher than previously reported). Similarly, 33 mg/L of retinoids was produced in flask by reconstructing apocarotenoid module, demonstrating the versatility of the "plug-n-play" modular system. Collectively, this study highlights the importance of the strategy of simultaneous modular pathway optimization and enzyme engineering to overproduce valuable chemicals in ...Continue Reading

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Citations

Oct 21, 2019·Applied Microbiology and Biotechnology·Li-Qun JinYu-Guo Zheng
May 14, 2020·Applied Microbiology and Biotechnology·Congqiang ZhangHeng-Phon Too
Nov 12, 2018·Journal of Industrial Microbiology & Biotechnology·Ramesh Kumar Saini, Young-Soo Keum
Mar 20, 2019·Microbial Cell Factories·Chonglong WangSeon-Won Kim
Sep 3, 2018·Microbial Cell Factories·Jeffrey J CzajkaYinjie J Tang
Jan 15, 2019·Critical Reviews in Food Science and Nutrition·K Srinivasan, Elna M Buys
May 28, 2019·Applied Microbiology and Biotechnology·Sandra Moser, Harald Pichler
Oct 27, 2020·Frontiers in Bioengineering and Biotechnology·Javiera LópezEduardo Agosin
Feb 13, 2020·Current Medicinal Chemistry·Congqiang Zhang, Heng-Phon Too
Feb 6, 2021·Critical Reviews in Biotechnology·Ming-Hua LiangJian-Guo Jiang
Jan 8, 2020·Metabolic Engineering Communications·Cheng LiAnthony J Sinskey
Apr 3, 2021·Critical Reviews in Food Science and Nutrition·Cassamo Ussemane MussagyAnna Maria Kot
May 1, 2021·Plants·Antonello PaparellaMwafaq Ibdah
Jul 8, 2021·Natural Product Reports·Mauro A RinaldiNigel S Scrutton
Dec 24, 2019·Journal of Agricultural and Food Chemistry·Xixian ChenNicholas D Lindley
Mar 14, 2019·Journal of Agricultural and Food Chemistry·Ilya LukinGerhard Schembecker
May 14, 2019·Journal of Agricultural and Food Chemistry·Xixian ChenCongqiang Zhang
Sep 24, 2021·Journal of Agricultural and Food Chemistry·Nan LiangYing-Jin Yuan
Oct 2, 2021·Journal of Agricultural and Food Chemistry·Nellie FrancezonLucie Beaulieu

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