Abstract
The point mutation serine 120 to glycine in the human nucleoside diphosphate kinase A has been identified in several aggressive neuroblastomas (Chang, C. L., Zhu, X. X., Thoraval, D. H., Ungar, D., Rawwas, J., Hora, N., Strahler, J. R., Hanash, S. M. & Radany, E. (1994) Nature 370, 335-336). We expressed in bacteria and purified wild-type and S120G mutant nucleoside diphosphate kinase A. The mutant enzyme had enzymatic and structural properties similar to the wild-type enzyme, whereas its stability to denaturation by heat and urea was markedly reduced. More importantly, upon renaturation of the urea-denatured mutant protein, a folding intermediate accumulated, having the characteristics of a molten globule. It had no tertiary structure, as shown by near UV circular dichroism, whereas the secondary structure was substantially recovered. The hydrophobic probe 8-anilino-1-naphthalene sulfonate bound to the intermediate species with an increase in fluorescence intensity and a blue shift. The hydrodynamic size was between that expected for a folded and an unfolded monomer. Finally, electrophoresis in a transverse urea gradient displayed no renaturation curve, and the protein showed the tendency to aggregate at the lowest urea concen...Continue Reading
References
Apr 5, 1979·Journal of Molecular Biology·T E Creighton
Jan 1, 1991·Methods in Enzymology·T A KunkelJ McClary
Nov 9, 1989·Nature·A M RosengardP S Steeg
Nov 1, 1989·Analytical Biochemistry·S C Gill, P H von Hippel
Nov 16, 1987·FEBS Letters·G V SemisotnovO B Ptitsyn
Oct 1, 1988·Genes & Development·J BiggsA Shearn
Sep 1, 1988·Developmental Biology·C R DearolfA Shearn
Oct 20, 1985·Journal of Molecular Biology·S CraigR H Pain
Oct 3, 1983·European Journal of Biochemistry·I LascuI Proinov
Feb 6, 1995·FEBS Letters·V E Bychkova, O B Ptitsyn
Aug 1, 1995·Proceedings of the National Academy of Sciences of the United States of America·D VenturelliB Calabretta
Aug 25, 1995·Journal of Molecular Biology·P A WebbR L Williams
May 1, 1995·Nature Structural Biology·R N Sifers
Jan 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·A de la RosaP S Steeg
Jun 2, 1995·The Journal of Biological Chemistry·L JiL M Boxer
Dec 27, 1994·Biochemistry·B E JonesC R Matthews
Feb 7, 1995·Biochemistry·U Nath, J B Udgaonkar
Aug 4, 1994·Nature·C L ChangE Radany
Jul 23, 1993·Science·E H PostelC A Ferrone
Nov 15, 1995·European Journal of Biochemistry·M EngelC Welter
Nov 1, 1995·Trends in Biochemical Sciences·P J ThomasP L Pedersen
Jul 26, 1996·The Journal of Biological Chemistry·A GiartosioI Lascu
Dec 15, 1995·Structure·S MoréraJ Janin
Oct 11, 1996·The Journal of Biological Chemistry·N J MacDonaldP S Steeg
Apr 1, 1997·Human Genetics·L MilonM L Lacombe
Citations
Mar 14, 2002·The Journal of Comparative Neurology·Perrine BarraudMarc Landry
Apr 13, 2011·Naunyn-Schmiedeberg's Archives of Pharmacology·Florian GeorgescauldIoan Lascu
Sep 1, 2006·Journal of Bioenergetics and Biomembranes·Daniela Lombardi
Sep 1, 2006·Journal of Bioenergetics and Biomembranes·Ioan Lascu
Sep 1, 2006·Journal of Bioenergetics and Biomembranes·Marie-France GiraudAlain Dautant
Apr 24, 2009·Molecular and Cellular Biochemistry·Mathieu BoissanMarie-Lise Lacombe
Jul 16, 2003·Biochemical and Biophysical Research Communications·Young-In KimHansoo Lee
Nov 7, 2000·Cancer Genetics and Cytogenetics·G CipolliniM A Caligo
Nov 24, 2001·Trends in Genetics : TIG·P Csermely
Jan 8, 1999·Brain Research. Molecular Brain Research·S DabernatM Landry
Jul 24, 2002·Experimental Hematology·Roel WillemsDirk R Van Bockstaele
Nov 18, 2000·Gene·I Hunger-GlaserT Seebeck
Mar 30, 2001·European Journal of Biochemistry·M ErentI Lascu
Apr 21, 2010·Journal of Biochemistry·Yunru ChenSheng Xiong
Mar 26, 2013·PloS One·Florian GeorgescauldIoan Lascu
Mar 19, 2013·PloS One·Benoit J SmaggheCynthia Bamdad
Jun 28, 2014·Science·Mathieu BoissanPhilippe Chavrier
Feb 3, 2009·FEBS Letters·Florian GeorgescauldIoan Lascu
Jan 27, 2009·Journal of Cellular Biochemistry·Lorenza EgistelliLaura Cervoni
Nov 18, 2000·Experimental Cell Research·D RoymansH Slegers
Mar 6, 2007·Biochemical and Biophysical Research Communications·Qinghua ZhouQin Yang
Jun 23, 1998·American Journal of Human Genetics·M T Hartsough, P S Steeg
Aug 6, 2015·The Protein Journal·Yasushi YonezawaMasao Tokunaga
Jan 17, 2009·Biochimica Et Biophysica Acta·Kimberly A P MitchellAngela de S Otero
Mar 23, 2012·PLoS Genetics·Hermann BauerBernhard G Herrmann
Dec 22, 1999·Oncogene·A ReymondM Zollo
Apr 12, 2002·FEBS Letters·Vladimir N Uversky, Anthony L Fink
Aug 8, 2006·Journal of Korean Medical Science·Ju-Han LeeInsun Kim
Jun 26, 2001·Cell Death and Differentiation·D LombardiM G Paggi
Jan 28, 2006·Journal of Cellular Biochemistry·Laura CervoniAnna Giartosio
Oct 11, 2017·Laboratory Investigation; a Journal of Technical Methods and Pathology·Choon-Yee Tan, Christina L Chang
Mar 21, 1998·The Journal of Biological Chemistry·S MesnildreyM Véron
Jul 10, 1999·The Journal of Biological Chemistry·S SchaertlM Konrad
Feb 13, 2004·The Journal of Biological Chemistry·Deqin MaDavid M Kaetzel
Aug 23, 2000·The Journal of Biological Chemistry·P D Wagner, N D Vu
Dec 13, 2006·The Biochemical Journal·Iulia MocanIoan Lascu
Sep 20, 2020·International Journal of Molecular Sciences·Florian GeorgescauldAlain Dautant
Jul 25, 2020·Molecular and Cellular Biochemistry·Yuanjun LiYung H Wong
Aug 7, 2017·F1000Research·Diana ZalaMathieu Boissan
Aug 6, 2020·International Journal of Molecular Sciences·Yuuta ImotoYukio Fujiki
Jul 10, 2002·The Journal of Biological Chemistry·Melanie T HartsoughPatricia S Steeg
Aug 24, 2000·Biochemistry·E J SongK J Lee
Apr 18, 2001·Biochemistry·M BruneM R Webb
Mar 20, 1999·Biochimica Et Biophysica Acta·A S OteroP S Steeg
Jul 10, 2021·Biochimie·Pragati AgnihotriJ Venkatesh Pratap
May 13, 1999·Biochemistry·K K TuroverovI M Kuznetsova
May 16, 2002·Biochemistry·Bin Zhang, Zheng-yu Peng