A Point-of-Need infrared mediated PCR platform with compatible lateral flow strip for HPV detection

Biosensors & Bioelectronics
Wenjia LiuXianting Ding

Abstract

With the increasing need of monitoring the epidemiology of serious infectious diseases, food hygiene, food additives and pesticide residues, it is urgent to develop portable, easy-to-use, inexpensive and rapid molecular diagnostic tools. Herein, we demonstrate a prototype of IR mediated Conducting Oil and CarbOn Nanotube circUlaTing PCR (IR-COCONUT PCR) platform for nucleic acid amplification. The presented platform offers a new solution for miniaturized PCR instruments with non-contact heaters by using conducting oil and carbon nanotube as a medium in IR mediated PCR. This novel platform offers accurate and flexible control of temperature through the integration of PID (proportional-integral-derivative) algorithms to manipulate the duty cycle of the voltage signals of IR LED and a peristaltic pump. The ramping rate of the introduced platform in current study is 1.5°C/s for heating speed and -2.0°C/s for cooling speed. This platform fulfills 30 thermal cycles within 50min which is a match to the conventional bench-top PCR thermo cyclers. For demonstration purpose, human papillomavirus (HPV) patient cervical swab specimens were examined. Downstream lateral flow strip (LFS) was also developed to quantity the PCR products from the...Continue Reading

Citations

Sep 22, 2019·Sensors·José R EspinosaSergio M Durón
Aug 30, 2017·Micromachines·Ismail Hussain Kamal BashaNor Hisham Bin Hamid
Dec 16, 2018·Biosensors & Bioelectronics·Sang Hun LeeLuke P Lee
Jul 1, 2021·Trends in Analytical Chemistry : TRAC·Xiaobin DongXianbo Qiu
Jul 14, 2021·Chemical Reviews·Min LiQiang Xia

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