A polarity dependent fluorescence "switch" in live cells

Journal of Photochemistry and Photobiology. B, Biology
Michael W BernsP M Rentzepis

Abstract

The spectroscopic properties, ultrafast kinetics and utilization of a photochromic molecule as a bi-stable fluorescing sensor of polarity in live cells are described. This molecule is a photochromic fulgimide, 2,3-dialkylidenesuccinimide, which emits fluorescence that can be switched optically on and off. The fluorescence intensity is a function of the polarity of the molecular environment, namely it fluoresces strongly when the molecule is in its polar isomeric structure form. We demonstrate that this molecule enters live cells without inducing damage, it binds primarily to internal membranous organelles (mitochondria) and its fluorescence can be switched optically "on" and "off" repeatedly while inside the living cell. A possible use as a bi-stable, on/off sensor is discussed.

References

Apr 6, 1990·Science·W DenkW W Webb
Aug 16, 2000·Proceedings of the National Academy of Sciences of the United States of America·M W BernsV Venugopalan

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Citations

Jun 15, 2006·Analytical Chemistry·Kristin A FletcherIsiah M Warner
Aug 1, 2009·The Journal of Organic Chemistry·Xi ChenWatson J Lees
Mar 8, 2011·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Xi ChenWatson J Lees
Mar 30, 2016·Physical Chemistry Chemical Physics : PCCP·C SlavovM Braun
Dec 7, 2007·Chemical Communications : Chem Comm·Nobuaki SohMasahiro Irie
Jan 26, 2012·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Kentaro KudoTatsuo Arai
Sep 13, 2011·Chemical Communications : Chem Comm·Jesper R NilssonJoakim Andréasson

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