PMID: 9426293Feb 28, 1998Paper

A polarized salivary cell monolayer useful for studying transepithelial fluid movement in vitro

Pflügers Archiv : European journal of physiology
X HeB J Baum

Abstract

There are no reported, convenient in vitro models for studying polarized functions in salivary epithelial cells. Accordingly, we examined three often-used salivary cell lines for their ability to form a polarized monolayer on permeable, collagen-coated polycarbonate filters. Only the SMIE line, derived from rat submandibular gland, had this ability. The SMIE cell monolayer exhibited junctional complexes, with a tight-junction-associated protein, ZO-1, localized to cell-cell contact areas. The Na+/K+-ATPase alpha1-subunit was detected predominantly in the basolateral membranes, while the Na+/H+ exchanger isoform 2 appeared primarily in the apical membranes. Using adenovirus-mediated cDNA transfer, SMIE cells were shown to be capable of routing marker proteins (beta-galactosidase +/- a nuclear targeting signal, alpha1-antitrypsin, aquaporin-1) to appropriate locations. Furthermore, this salivary cell monolayer provided a convenient tool for studying aquaporin-1-mediated, osmotically directed, transepithelial fluid movement in vitro. Thus, SMIE cells appear to be a useful experimental model with which to study some polarized functions in a salivary epithelial cell line.

Citations

Aug 15, 2013·Cell and Tissue Research·Ubaidus SobhanYoshiyuki Shibukawa
Apr 5, 2001·Archives of Oral Biology·T MurataT Tagawa
Sep 21, 2000·Proceedings of the Society for Experimental Biology and Medicine·R CastroJ R Martinez
Jul 11, 2012·The Journal of Biological Chemistry·Bidhan C BandyopadhyayIndu S Ambudkar
Dec 10, 1999·Human Gene Therapy·B J BaumJ Chao
Feb 26, 2010·Journal of Biomedicine & Biotechnology·Olga J Baker
Feb 2, 2016·International Journal of Molecular Sciences·Christine DelporteJason Perret
Oct 26, 2010·International Journal of Radiation Oncology, Biology, Physics·Arjan VissinkMary E Reyland
Sep 2, 2016·Tissue Barriers·Olga J Baker
Dec 22, 2005·Biochimica Et Biophysica Acta·Bruce J BaumGabor G Illei
Mar 16, 2006·Biochimica Et Biophysica Acta·Christine Delporte, Serge Steinfeld
Aug 17, 2005·Archives of Oral Biology·Kasumi ShimizuToshiro Tagawa
Aug 21, 2013·Biochimica Et Biophysica Acta·Christine Delporte
Apr 12, 2000·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·J T TurnerB R Talamo
May 16, 2002·Journal of Cellular Physiology·A T M Shamsul HoqueBruce J Baum
Dec 30, 2004·The Journal of Biological Chemistry·Bidhan C BandyopadhyayIndu S Ambudkar
Mar 28, 2001·The Journal of Gene Medicine·L BaccagliniB J Baum
Jun 8, 2017·Frontiers in Physiology·Ryouichi SatouYoshiyuki Shibukawa
Sep 16, 2018·International Journal of Molecular Sciences·Imran NizamuddinCarole P McArthur
Jun 10, 1998·Biochemical and Biophysical Research Communications·C DelporteB J Baum

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