A polyketide synthase gene required for biosynthesis of the aflatoxin-like toxin, dothistromin.

Mycopathologia
Rosie E BradshawShuguang Zhang

Abstract

Dothistromin is a polyketide toxin, produced by a fungal forest pathogen, with structural similarity to the aflatoxin precursor versicolorin B. Biochemical and genetic studies suggested that there are common steps in the biosynthetic pathways for these metabolites and showed similarities between some of the genes. A polyketide synthase gene (pksA) was isolated from dothistromin-producing Dothistroma septosporum by hybridization with an aflatoxin ortholog from Aspergillus parasiticus. Inactivation of this gene in D. septosporum resulted in mutants that could not produce dothistromin but that could convert exogenous aflatoxin precursors, including norsolorinic acid, into dothistromin. The mutants also had reduced asexual sporulation compared to the wild type. So far four other genes are known to be clustered immediately alongside pksA. Three of these (cypA, moxA, avfA) are predicted to be orthologs of aflatoxin biosynthetic genes. The other gene (epoA), located between avfA and moxA, is predicted to encode an epoxide hydrolase, for which there is no homolog in either the aflatoxin or sterigmatocystin gene clusters. The pksA gene is located on a small chromosome of approximately 1.3 Mb in size, along with the dothistromin ketoredu...Continue Reading

References

Feb 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·D W BrownT J Leonard
Sep 1, 1996·Applied and Environmental Microbiology·S P KaleJ W Bennett
Feb 1, 1997·Fungal Genetics and Biology : FG & B·N P Keller, T M Hohn
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Jun 26, 1999·Journal of Food Protection·J W DornerD T Wicklow
Nov 10, 2001·Annual Review of Phytopathology·U Liane Rosewich, H Corby Kistler
Dec 26, 2001·Toxicological Sciences : an Official Journal of the Society of Toxicology·Edward J KellyDavid L Eaton
Jun 1, 2002·Applied and Environmental Microbiology·Rosie E BradshawJanet M Seconi
Mar 26, 2003·Applied Microbiology and Biotechnology·D BhatnagarT E Cleveland
Jul 15, 2003·Clinical Microbiology Reviews·J W Bennett, M Klich
Dec 17, 2003·Proceedings of the National Academy of Sciences of the United States of America·Scott KrokenB Gillian Turgeon
Mar 10, 2004·Applied and Environmental Microbiology·Jiujiang YuJoan W Bennett
Apr 20, 2004·FEBS Letters·Jiujiang YuThomas E Cleveland
Jun 4, 2005·Applied and Environmental Microbiology·Ying WenKimiko Yabe

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Citations

Apr 2, 2010·Natural Product Reports·Hui ZhouYi Tang
Dec 1, 2009·Toxins·Rosie E BradshawShuguang Zhang
Nov 10, 2011·Toxins·Arne Schwelm, Rosie E Bradshaw
Oct 31, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jason M CrawfordCraig A Townsend
Dec 19, 2012·Annual Review of Food Science and Technology·Ludmila V RozeJohn E Linz
Dec 5, 2012·Fungal Genetics and Biology : FG & B·Pranav ChettriRosie E Bradshaw
Jul 23, 2011·Journal of Microbiological Methods·Rebecca McDougalRosie Bradshaw
Jan 25, 2008·Bioorganic Chemistry·Jason M CrawfordCraig A Townsend
Jan 10, 2012·Fungal Genetics and Biology : FG & B·Pranav ChettriRosie E Bradshaw
Mar 29, 2007·Natural Product Reports·Dirk Hoffmeister, Nancy P Keller
Nov 8, 2016·Fungal Genetics and Biology : FG & B·Pranav Chettri, Rosie E Bradshaw
Sep 1, 2006·Mycopathologia·Rosie E Bradshaw, Shuguang Zhang
Jul 30, 2020·Scientific Reports·Somsiri UdompaisarnAlongkorn Amnuaykanjanasin
Nov 16, 2019·Fungal Genetics and Biology : FG & B·Yanan GuoRosie E Bradshaw

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