A polymer surfactant corona dynamically replaces water in solvent-free protein liquids and ensures macromolecular flexibility and activity

Journal of the American Chemical Society
François-Xavier GallatM Weik

Abstract

The observation of biological activity in solvent-free protein-polymer surfactant hybrids challenges the view of aqueous and nonaqueous solvents being unique promoters of protein dynamics linked to function. Here, we combine elastic incoherent neutron scattering and specific deuterium labeling to separately study protein and polymer motions in solvent-free hybrids. Myoglobin motions within the hybrid are found to closely resemble those of a hydrated protein, and motions of the polymer surfactant coating are similar to those of the hydration water, leading to the conclusion that the polymer surfactant coating plasticizes protein structures in a way similar to hydration water.

References

Dec 2, 1975·Biochemistry·R H AustinI C Gunsalus
Dec 13, 1991·Science·H FrauenfelderP G Wolynes
Mar 21, 2000·Nature·A OstermannG U Nienhaus
Feb 24, 2001·Nature·A M Klibanov
Mar 8, 2003·Quarterly Reviews of Biophysics·Frank GabelGiuseppe Zaccai
May 17, 2005·Biochimica Et Biophysica Acta·Wolfgang Doster, Marcus Settles
Aug 11, 2005·Physical Review Letters·J H RohA P Sokolov
Dec 22, 2007·Chemical Reviews·Philip Ball
Mar 3, 2009·Proceedings of the National Academy of Sciences of the United States of America·Hans FrauenfelderRobert D Young
Jul 16, 2009·Angewandte Chemie·Adam W PerrimanStephen Mann
Jan 14, 2010·Journal of the American Chemical Society·Giorgio SchiróAntonio Cupane
Apr 8, 2010·Biophysical Journal·S KhodadadiA P Sokolov
Jul 24, 2010·Nature Chemistry·Adam W PerrimanStephen Mann
Jul 14, 2011·ACS Nano·Adam W Perriman, Stephen Mann
Aug 3, 2012·The Review of Scientific Instruments·Joachim WuttkeSimon Staringer

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Citations

Oct 7, 2014·Nature Communications·Alex P S BroganStephen Mann
Mar 16, 2016·Journal of the American Chemical Society·Alex P S Brogan, Jason P Hallett
May 2, 2015·Science·Józef R LewandowskiLyndon Emsley
Sep 22, 2016·Biomacromolecules·Thomas A G RisbridgerDavid J Fermin
Oct 17, 2017·Nature Communications·Samuel LentonLorna Dougan
Jan 10, 2019·Chembiochem : a European Journal of Chemical Biology·Ye ZhouZheng Guo
Jul 6, 2019·Physical Chemistry Chemical Physics : PCCP·Zhaohui HuangJingcheng Hao
Oct 18, 2019·Nanoscale·Dylan Luke AtkinsIlja Karina Voets
May 28, 2016·Experimental Biology and Medicine·James Pk Armstrong, Adam W Perriman
Aug 6, 2019·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Giorgio Schirò, Martin Weik
Aug 23, 2019·Chemical Communications : Chem Comm·Alex P S BroganAngela M Belcher
Aug 18, 2018·Scientific Reports·Bianca PérezBirgit Schiøtt
Oct 23, 2020·Biotechnology and Bioengineering·Liem Bui-LeJason P Hallett
Mar 13, 2021·Physical Review Letters·Giorgio SchiròMartin Weik
Apr 21, 2021·Physical Chemistry Chemical Physics : PCCP·Sudarshan BeheraSundaram Balasubramanian
Nov 20, 2013·Journal of the American Chemical Society·Kamendra P SharmaDavid J Fermin
Feb 13, 2014·Biomacromolecules·Nisaraporn SuthiwangcharoenQian Wang
Jan 25, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·D RussoJ Teixeira
Jun 25, 2013·The Journal of Physical Chemistry. B·Alex P S BroganStephen Mann

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