PMID: 8615447Apr 1, 1996Paper

A polymerase chain reaction assay for detection of the parasite Wuchereria bancrofti in human blood samples

The American Journal of Tropical Medicine and Hygiene
M ZhongS A Williams

Abstract

To identify Wuchereria bancrofti DNA sequences that could be used as the basis for a simple and rapid parasite detection assay, a genomic library of W. bancrofti was constructed and screened for highly repeated DNA. The repeat found with the highest copy number was 195 basepairs (bps) long, 77% AT, and 300 copies per haploid genome. This sequence was designated the Ssp I repeat because it has a unique recognition site for that restriction endonuclease in all or most of the repeat copies. The Ssp I repeat DNA family is dispersed, genus-specific, and exists in all of the different geographic isolates of W. bancrofti tested. Based on DNA sequence analysis of this repeat, we have developed an assay to detect very small quantities of W. bancrofti DNA using the polymerase chain reaction (PCR). With this PCR assay, the Ssp I repeat was detected in as little as 1 pg of w. bancrofti genomic DNA (about 1% of the DNA in one microfilaria) added to 100 microliters of human blood. The PCR assay also amplified Ssp I repeat DNA from geographic isolates of W. bancrofti from around the world but not from other species of filariae or from human or mosquito DNA. Microfilaria-positive human blood samples collected in Mauke, Cook Islands were shown ...Continue Reading

References

Apr 1, 1990·Molecular and Biochemical Parasitology·C B Poole, S A Williams
Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·L A McReynoldsS A Williams
Mar 1, 1988·Molecular and Biochemical Parasitology·S A WilliamsL A McReynolds
Nov 1, 1994·Transactions of the Royal Society of Tropical Medicine and Hygiene·S ChanteauS A Williams
Nov 1, 1995·Transactions of the Royal Society of Tropical Medicine and Hygiene·J P Moulia-PelatL Nicolas
Mar 1, 1996·Transactions of the Royal Society of Tropical Medicine and Hygiene·L NicolasD R Mercer
Jan 1, 1994·Parasitology Today·T B NutmanD D Kostyu

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Citations

Jan 31, 2006·Transactions of the Royal Society of Tropical Medicine and Hygiene·Taniawati SupaliPeter Fischer
Mar 25, 2000·International Journal for Parasitology·S A WilliamsM L Blaxter
Dec 12, 1997·International Journal for Parasitology·B Singh
Jan 2, 2001·Transactions of the Royal Society of Tropical Medicine and Hygiene·S DissanayakeW F Piessens
Mar 31, 2001·Transactions of the Royal Society of Tropical Medicine and Hygiene·H A FaridR M Ramzy
Jun 12, 2002·Transactions of the Royal Society of Tropical Medicine and Hygiene·Reda M R Ramzy
Mar 1, 1997·Transactions of the Royal Society of Tropical Medicine and Hygiene·R M RamzyA M Gad
Dec 16, 1998·Transactions of the Royal Society of Tropical Medicine and Hygiene·N RahmahS A Williams
Sep 13, 2002·Insect Biochemistry and Molecular Biology·L McCarroll, J Hemingway
Jan 5, 2000·Microbes and Infection·A B de Almeida, D O Freedman
May 7, 2013·Asian Pacific Journal of Tropical Biomedicine·Tularat SooktoBinit Shrestha
Jan 14, 2010·Interdisciplinary Perspectives on Infectious Diseases·Momar Ndao
Jul 10, 1998·Tropical Medicine & International Health : TM & IH·F S TouréT G Egwang
Sep 8, 2006·Journal of Clinical Microbiology·Ramakrishna U RaoPeter Fischer
Dec 1, 1997·Indian Journal of Clinical Biochemistry : IJCB· LalithaR B Narayanan
Dec 23, 1998·Transactions of the Royal Society of Tropical Medicine and Hygiene·W A LucenaA F Furtado
Oct 13, 2009·Expert Review of Molecular Diagnostics·Shuzhen SimGeorge Dimopoulos
Jun 4, 2010·The American Journal of Tropical Medicine and Hygiene·Rajeev K MehlotraPeter A Zimmerman
Dec 29, 2012·PLoS Neglected Tropical Diseases·Catherine B PooleClotilde K S Carlow
Jul 1, 1997·Cadernos de saúde pública·G Dreyer, G Coelho
Apr 6, 2005·Tropical Medicine & International Health : TM & IH·Aiah A GbakimaAlan L Scott
Feb 18, 2005·Revista Da Sociedade Brasileira De Medicina Tropical·Janaina MirandaElizabeth Malagueno
Mar 11, 2003·Annals of Tropical Medicine and Parasitology·R M R Ramzy
Jun 7, 2013·Revista Da Sociedade Brasileira De Medicina Tropical·Rodolfo Luís KorteLuís Marcelo Aranha Camargo
Jul 1, 1996·Transactions of the Royal Society of Tropical Medicine and Hygiene·S A WilliamsJ P Moulia-Pelat
Aug 15, 2017·Case Reports in Infectious Diseases·Jean-Marc Chavatte, Roland Jureen

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