A Polymorphism in the Processing Body Component Ge-1 Controls Resistance to a Naturally Occurring Rhabdovirus in Drosophila

PLoS Pathogens
Chuan CaoFrancis M Jiggins

Abstract

Hosts encounter an ever-changing array of pathogens, so there is continual selection for novel ways to resist infection. A powerful way to understand how hosts evolve resistance is to identify the genes that cause variation in susceptibility to infection. Using high-resolution genetic mapping we have identified a naturally occurring polymorphism in a gene called Ge-1 that makes Drosophila melanogaster highly resistant to its natural pathogen Drosophila melanogaster sigma virus (DMelSV). By modifying the sequence of the gene in transgenic flies, we identified a 26 amino acid deletion in the serine-rich linker region of Ge-1 that is causing the resistance. Knocking down the expression of the susceptible allele leads to a decrease in viral titre in infected flies, indicating that Ge-1 is an existing restriction factor whose antiviral effects have been increased by the deletion. Ge-1 plays a central role in RNA degradation and the formation of processing bodies (P bodies). A key effector in antiviral immunity, the RNAi induced silencing complex (RISC), localises to P bodies, but we found that Ge-1-based resistance is not dependent on the small interfering RNA (siRNA) pathway. However, we found that Decapping protein 1 (DCP1) protec...Continue Reading

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Citations

Jan 20, 2017·Cellular and Molecular Life Sciences : CMLS·Assel MussabekovaJean-Luc Imler
Jul 28, 2016·Molecular Ecology·Rodrigo CogniFrancis M Jiggins
Mar 10, 2018·Viruses·William H PalmerRonald P van Rij
Dec 2, 2020·Molecular Biology and Evolution·Camille AmelineDieter Ebert

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Methods Mentioned

BETA
PCR
transgenic
genotyping
PCRs

Software Mentioned

VMA2FASTA
MASS ”
MCMCglmm
R library
R
FastA
BioEdit

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