A Polyphasic and Taxogenomic Evaluation Uncovers Arcobacter cryaerophilus as a Species Complex That Embraces Four Genomovars

Frontiers in Microbiology
Alba Pérez-CataluñaMaría José Figueras

Abstract

The species Arcobacter cryaerophilus is found in many food products of animal origin and is the dominating species in wastewater. In addition, it is associated with cases of farm animal and human infectious diseases,. The species embraces two subgroups i.e., 1A (LMG 24291T = LMG 9904T) and 1B (LMG 10829) that can be differentiated by their 16S rRNA-RFLP pattern. However, some authors, on the basis of the shared intermediate levels of DNA-DNA hybridization, have suggested abandoning the subgroup classification. This contradiction indicates that the taxonomy of this species is not yet resolved. The objective of the present study was to perform a taxonomic evaluation of the diversity of A. cryaerophilus. Genomic information was used along with a Multilocus Phylogenetic Analysis (MLPA) and phenotypic characterization on a group of 52 temporally and geographically dispersed strains, coming from different types of samples and hosts from nine countries. The MLPA analysis showed that those strains formed four clusters (I-IV). Values of Average Nucleotide Identity (ANI) and in silico DNA-DNA Hybridization (isDDH) obtained between 13 genomes representing strains of the four clusters were below the proposed cut-offs of 96 and 70%, respect...Continue Reading

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Citations

May 12, 2020·International Journal of Systematic and Evolutionary Microbiology·Stephen L W OnPeter Vandamme
Sep 21, 2018·Frontiers in Microbiology·Alba Pérez-CataluñaMaria J Figueras

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Datasets Mentioned

BETA
7299

Methods Mentioned

BETA
glycosylation

Software Mentioned

RAST
VF
Specialty Genes
HMMER
MEGA
Rapid Annotation Subsystem Technology ( RAST )
PATRIC
BLASTp
ANNOT
Seed Viewer

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