PMID: 7539420Jun 1, 1995Paper

A polypurine sequence that acts as a 5' mRNA stabilizer in Bacillus subtilis

Journal of Bacteriology
K HueD H Bechhofer

Abstract

A segment of early RNA from Bacillus subtilis bacteriophage SP82 was shown to function as a 5' stabilizer in B. subtilis. Several heterologous RNA sequences were stabilized by the presence of the SP82 sequence at the 5' end, and expression of downstream coding sequences was increased severalfold. The SP82 RNA segment encodes a B. subtilis RNase III cleavage site, but cleavage by B. subtilis RNase III was not required for stabilization. The sequence that specifies 5' stabilizer function was localized to a polypurine sequence that resembles a ribosome binding site. The ability of the SP82 sequence to stabilize downstream RNA was dependent on its position relative to the 5' end of the RNA. These results demonstrate the existence of a new type of 5' stabilizer in B. subtilis and indicate that attack at the 5' end is a principal mechanism for initiation of mRNA decay in B. subtilis.

References

Jan 1, 1992·Genes & Development·S A EmoryJ G Belasco
Mar 5, 1991·Journal of Molecular Biology·D HartzL Gold
Jun 1, 1991·Journal of Bacteriology·K K Hue, D H Bechhofer
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S G GrantD Hanahan
Jun 26, 1990·Biochemistry·C O Gualerzi, C L Pon
Nov 1, 1989·Journal of Bacteriology·D H Bechhofer, K H Zen
Oct 1, 1989·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J L Hargrove, F H Schmidt
Sep 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S AltuviaA B Oppenheim
Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·D H Bechhofer, D Dubnau
Jan 1, 1987·Methods in Enzymology·T A KunkelR A Zakour
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·A G ShivakumarD Dubnau
Jan 1, 1984·Molecular & General Genetics : MGG·T J GryczanD Dubnau
Jul 1, 1983·Cell·A T Panganiban, H R Whiteley
Mar 1, 1993·Molecular Microbiology·J F DiMari, D H Bechhofer

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Citations

Oct 6, 2020·Frontiers in Microbiology·Diego A Vargas-Blanco, Scarlet S Shell
Jun 26, 1999·Molecular Microbiology·G HomuthW Schumann
Mar 29, 2002·Molecular Microbiology·Djamel DriderDavid H Bechhofer
Feb 13, 1999·Molecular Microbiology·S J StasinopoulosD H Bechhofer
Sep 1, 2005·The Journal of Biological Chemistry·Emma RedonMuriel Cocaign-Bousquet
Jul 29, 2003·Molecular Genetics and Genomics : MGG·G HambraeusL Hederstedt
Sep 23, 2006·FEMS Microbiology Reviews·Vladimir R Kaberdin, Udo Bläsi
Sep 26, 2013·Cellular and Molecular Life Sciences : CMLS·Soumaya LaalamiHarald Putzer
Dec 1, 2011·Biomolecular Concepts·Soumaya Laalami, Harald Putzer
Jun 28, 2005·Molecular Microbiology·Josh S Sharp, David H Bechhofer
Dec 26, 2006·Journal of Bacteriology·Timothy C BarnettJune R Scott
Sep 2, 2003·Journal of Bacteriology·Josh S Sharp, David H Bechhofer
Jan 30, 1999·Applied and Environmental Microbiology·U PagH G Sahl
Jan 14, 2000·Journal of Bacteriology·M PerssonB Rutberg
Jun 10, 2003·Microbiology and Molecular Biology Reviews : MMBR·Ciarán Condon
Nov 13, 1998·Journal of Bacteriology·D H Bechhofer, W Wang
Sep 15, 1998·Journal of Molecular Biology·S A Joyce, M Dreyfus
Apr 1, 1996·Journal of Bacteriology·W Wang, D H Bechhofer
Oct 23, 1997·Journal of Bacteriology·C Vázquez-Cruz, G Olmedo-Alvarez
Jul 1, 1997·Applied and Environmental Microbiology·A GuerchicoffC P Rubinstein
Nov 1, 1995·Journal of Bacteriology·H Agaisse, D Lereclus
Jul 7, 2007·Molecular Cell·Helena CelesnikJoel G Belasco

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