A polysaccharide-protein complex from Haemophilus influenzae type b. II. Human antibodies to its somatic components

The Journal of Infectious Diseases
R A InselD H Smith

Abstract

A polysaccharide-protein complex prepared from Haemophilus influenzae type b strain Eagan was used as test antigen in an enzyme-linked immunosorbent assay for human serum antibodies. With washing buffer that did not contain detergent, the assay detected antibody to lipopolysaccharide (LPS) and to non-LPS somatic antigens as well as to polyribosylribitolphosphate (PRP. With buffer that did contain detergent, antibodies to LPS were not detected, whereas detection of antibodies to the non-LPS somatic components and to PRP wa unimpeded. Similarly, purified LPS could be used as test antigen with the former buffer only. IgG, IgA, and IgM antibodies to non-LPS somatic antigens were prevalent in healthy adults and children, and levels increased in 14 of 15 patients recovering from meningitis due to H. influenzae type b; IgG was the predominant class. Antibodies to LPS were prevalent but at lower concentrations, and IgM was the predominant class; levels increased in 12 of the 15 patients.

Citations

Dec 1, 1982·The Journal of Clinical Investigation·F Gigliotti, R A Insel
Apr 1, 1987·Scandinavian Journal of Dental Research·K SchenckT Tollefsen
Mar 1, 1983·Journal of Clinical Microbiology·T Dahlberg-Lagergård

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