A poly(thymine)-templated fluorescent copper nanoparticle hydrogel-based visual and portable strategy for an organophosphorus pesticide assay

The Analyst
Jihua ChenXiaojun Zhang

Abstract

Since fluorescence assays with high sensitivity for organophosphorus pesticides (OPs) are urgently required to protect the ecosystem and prevent disease, an environmentally friendly and label-free fluorescent probe is desirable. Herein, a poly-thymine30 DNA-templated copper nanoparticle (poly T30-Cu NPs) hydrogel fluorescent probe was explored for the construction of an OPs sensing platform via tyrosinase (TYR) enzyme-controlled quenching. Initially, TYR can efficiently quench the fluorescence of poly T30-Cu NPs; however, when OPs are mixed with a certain amount of TYR, the fluorescence of poly T30-Cu NPs can be recovered. Based on this phenomenon, we designed a functionalized hydrogel based on poly T30-Cu NPs for portable and visible detection of OPs with high sensitivity and selectivity. This proposed fluorescent platform was demonstrated to enable rapid detection of OPs (paraoxon as the model analyte) and provide excellent sensitivity with a detection limit of 3.33 × 10-5 ng μL-1 and a linear range of 1.0 × 10-4-1.0 ng μL-1. The fluorescent probe does not require a sophisticated synthesis and labeling process; in addition, it is environmentally friendly because of the presence of a biotemplate of DNA and biocompatible copper...Continue Reading

References

Aug 29, 2006·Chemical Society Reviews·Iryna A KovalJan Reedijk
May 1, 2008·Journal of the American Chemical Society·Huanghao YangWeihong Tan
Oct 15, 2009·Journal of the American Chemical Society·Bin-Cheng YinCong-Cong Xie
Jul 16, 2010·Angewandte Chemie·Alexandru RotaruAndriy Mokhir
Nov 17, 2010·ACS Applied Materials & Interfaces·Ajfan BaeissaJuewen Liu
Dec 4, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Huaizhi KangWeihong Tan
Apr 4, 2012·ACS Applied Materials & Interfaces·Youssef HelwaJuewen Liu
Sep 25, 2015·Chemical Society Reviews·Yu TaoXiaogang Qu

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Methods Mentioned

BETA
biosensors
transmission electron microscopy
biosensing
Fluorescence

Software Mentioned

FL Solution

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