A Population Study of Common Ocular Abnormalities in C57BL/6N rd8 Mice

Investigative Ophthalmology & Visual Science
Bret A MooreAla Moshiri

Abstract

The purpose of this study was to quantify the frequency and severity of ocular abnormalities affecting wild-type C57BL/6N mice, the most common strain used worldwide for the creation of single-gene knockouts. A total of 2773 animals (5546 eyes) were examined at one colony at UC Davis and in three more colonies at the Institut Clinique de la Souris in Strasbourg, France. Mice were examined at 15 to 16 weeks postnatal age by performing anterior segment biomicroscopy, posterior segment examination by indirect ophthalmoscopy, intraocular pressure measurement, and optical coherence tomography of anterior and posterior segment structures. Common ocular findings in the C57BL/6N strain included corneal deposits (3%), increased optical density of the anterior lens capsule (67%), punctate nuclear cataracts (98%), vitreous crystalline deposits (61%), hyaloid vascular remnant (6%), and retinal dysplasia attributed to the rd8 mutation (58%). Interestingly, retinal dysplasia was more common in male mice in all four breeding colonies evaluated in this study. The thickness of ocular tissues and compartments were measured by spectral-domain optical coherence tomography, including the central cornea, anterior chamber, vitreous, and retinal layer...Continue Reading

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Citations

Sep 29, 2019·Pigment Cell & Melanoma Research·Jessica L FlesherAnand K Ganesan
Sep 12, 2020·Investigative Ophthalmology & Visual Science·Vincent LhussiezRomain Da Costa
Apr 17, 2020·Frontiers in Aging Neuroscience·Yuki IshimaruSadaaki Maeda
Dec 16, 2020·Disease Models & Mechanisms·Morag A LewisKaren P Steel
Oct 22, 2019·Developmental Biology·Young Sam KwonAla Moshiri
Jun 23, 2021·Investigative Ophthalmology & Visual Science·Renita Maria MartisJulie Ching-Hsia Lim

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