A porphyrin-based fluorescence method for zinc determination in commercial propolis extracts without sample pretreatment

Luminescence : the Journal of Biological and Chemical Luminescence
Gastón Darío PieriniMaría Susana Di Nezio

Abstract

The quantification of zinc in over-the-counter drugs as commercial propolis extracts by molecular fluorescence technique using meso-tetrakis(4-carboxyphenyl)porphyrin (H2 TCPP4 ) was developed for the first time. The calibration curve is linear from 6.60 to 100 nmol L-1 of Zn2+ . The detection and quantification limits were 6.22 nmol L-1 and 19.0 nmol L-1 , respectively. The reproducibility and repeatability calculated as the percentage variation of slopes of seven calibration curves were 6.75% and 4.61%, respectively. Commercial propolis extract samples from four Brazilian states were analyzed and the results (0.329-0.797 mg/100 mL) obtained with this method are in good agreement with that obtained with the Atomic Absorption Spectroscopy (AAS) technique. The method is simple, fast, of low cost and allows the analysis of the samples without pretreatment. Moreover the major advantage is that Zn-porphyrin complex presents fluorescent characteristic promoting the selectivity and sensitivity of the method.

References

Jun 28, 1972·Journal of the American Chemical Society·R F PasternackL de C Hinds
Jul 3, 1998·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·G A Burdock
Nov 30, 2007·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Júlio S RebouçasInes Batinić-Haberle
Oct 31, 2008·Talanta·M BiesagaM Trojanowicz
Aug 8, 2009·Journal of Pharmaceutical and Biomedical Analysis·Júlio S RebouçasInes Batinić-Haberle
May 28, 2015·BMC Complementary and Alternative Medicine·Loreta KubilieneArunas Savickas
May 28, 2015·Luminescence : the Journal of Biological and Chemical Luminescence·Wen-Tong ChenYun-Peng Pei
Feb 3, 2016·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Krzysztof KilianKrystyna Pyrzyńska

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