A Porphyromonas gingivalis mutant defective in a putative glycosyltransferase exhibits defective biosynthesis of the polysaccharide portions of lipopolysaccharide, decreased gingipain activities, strong autoaggregation, and increased biofilm formation.

Infection and Immunity
Mikiyo YamaguchiKoji Nakayama

Abstract

The Gram-negative anaerobic bacterium Porphyromonas gingivalis is a major pathogen in periodontal disease, one of the biofilm-caused infectious diseases. The bacterium possesses potential virulence factors, including fimbriae, proteinases, hemagglutinin, lipopolysaccharide (LPS), and outer membrane vesicles, and some of these factors are associated with biofilm formation; however, the precise mechanism of biofilm formation is still unknown. Colonial pigmentation of the bacterium on blood agar plates is related to its virulence. In this study, we isolated a nonpigmented mutant that had an insertion mutation within the new gene PGN_1251 (gtfB) by screening a transposon insertion library. The gene shares homology with genes encoding glycosyltransferase 1 of several bacteria. The gtfB mutant was defective in biosynthesis of both LPSs containing O side chain polysaccharide (O-LPS) and anionic polysaccharide (A-LPS). The defect in the gene resulted in a complete loss of surface-associated gingipain proteinases, strong autoaggregation, and a marked increase in biofilm formation, suggesting that polysaccharide portions of LPSs influence attachment of gingipain proteinases to the cell surface, autoaggregation, and biofilm formation of P...Continue Reading

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Citations

Aug 2, 2013·International Journal of Molecular Sciences·Pablo C BoginoWalter Giordano
Jun 13, 2015·Medical Microbiology and Immunology·Arbia KhemiriPascal Cosette
Oct 13, 2015·Microbial Pathogenesis·Mikio Shoji, Koji Nakayama
Jan 3, 2013·Molecular Oral Microbiology·A W AruniH M Fletcher
May 11, 2013·European Journal of Oral Sciences·Mikiyo YamaguchiShigeyuki Ebisu
Nov 23, 2011·Environmental Microbiology·Yosuke TashiroNobuhiko Nomura
Jun 17, 2015·BioMed Research International·Hideo YonezawaShigeru Kamiya
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Dec 20, 2017·Journal of Food Protection·Woo-Ju KimDong-Hyun Kang
Jan 10, 2021·Scientific Reports·Maki SotozonoShigeyuki Ebisu

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