A portable approach for the surveillance of dengue virus-infected mosquitoes

Journal of Virological Methods
David A MullerPaul Young

Abstract

Dengue virus is the most significant human viral pathogen spread by the bite of an infected mosquito. With no vaccine or antiviral therapy currently available, disease prevention relies largely on surveillance and mosquito control. Preventing the onset of dengue outbreaks and effective vector management would be considerably enhanced through surveillance of dengue virus prevalence in natural mosquito populations. However, current approaches to the identification of virus in field-caught mosquitoes require relatively slow and labor intensive techniques such as virus isolation or RT-PCR involving specialized facilities and personnel. A rapid and portable method for detecting dengue virus-infected mosquitoes is described. Using a hand held battery operated homogenizer and a dengue diagnostic rapid strip the viral protein NS1 was detected as a marker of dengue virus infection. This method could be performed in less than 30 min in the field, requiring no downstream processing, and is able to detect a single infected mosquito in a pool of at least 50 uninfected mosquitoes. The method described in this study allows rapid, real-time monitoring of dengue virus presence in mosquito populations and could be a useful addition to effective ...Continue Reading

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Citations

Nov 28, 2012·The American Journal of Tropical Medicine and Hygiene·Natalia V VogeBarry J Beaty
Jan 24, 2014·BioMed Research International·Ravi Kant Upadhyay
Jun 26, 2021·Frontiers in Medicine·Triwibowo Ambar GarjitoRoger Frutos
Aug 19, 2021·Journal of the American Mosquito Control Association·Kristen L Burkhalter, Harry M Savage
May 29, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Julia A WalkerSimon R Corrie

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