A portable automatic endpoint detection system for amplicons of loop mediated isothermal amplification on microfluidic compact disk platform

Sensors
Shah Mukim UddinKwai Lin Thong

Abstract

In recent years, many improvements have been made in foodborne pathogen detection methods to reduce the impact of food contamination. Several rapid methods have been developed with biosensor devices to improve the way of performing pathogen detection. This paper presents an automated endpoint detection system for amplicons generated by loop mediated isothermal amplification (LAMP) on a microfluidic compact disk platform. The developed detection system utilizes a monochromatic ultraviolet (UV) emitter for excitation of fluorescent labeled LAMP amplicons and a color sensor to detect the emitted florescence from target. Then it processes the sensor output and displays the detection results on liquid crystal display (LCD). The sensitivity test has been performed with detection limit up to 2.5 × 10(-3) ng/µL with different DNA concentrations of Salmonella bacteria. This system allows a rapid and automatic endpoint detection which could lead to the development of a point-of-care diagnosis device for foodborne pathogens detection in a resource-limited environment.

References

Feb 5, 1998·Journal of Applied Microbiology·R F WangC E Cerniglia
Jan 7, 2000·Annals of Biomedical Engineering·J J PancrazioD A Stenger
Jun 28, 2000·Nucleic Acids Research·T NotomiT Hase
Feb 28, 2001·Fresenius' Journal of Analytical Chemistry·C Ziegler
Feb 28, 2001·Fresenius' Journal of Analytical Chemistry·A WarsinkeF W Scheller
Sep 28, 2001·Biochemical and Biophysical Research Communications·J G BrunoC A Batt
Jun 25, 2002·Veterinary Microbiology·S D OliveiraC W Canal
May 20, 2004·International Journal of Medical Microbiology : IJMM·Irina Majoul
Dec 3, 2004·Applied and Environmental Microbiology·Burkhard MalornyReiner Helmuth
Apr 28, 2005·Lab on a Chip·M GrumannJ Ducrée
Jul 26, 2005·Biosensors & Bioelectronics·Sungho Ko, Sheila A Grant
Jul 13, 2006·Annual Review of Biomedical Engineering·Marc MadouNahui Kim
Dec 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Christopher J EasleyJames P Landers
Aug 24, 2007·Lab on a Chip·Stefan Haeberle, Roland Zengerle
Feb 23, 2008·Advances in Food and Nutrition Research·Arun K Bhunia
Aug 5, 2008·Foodborne Pathogens and Disease·Avraham Rasooly, Keith E Herold
Nov 22, 2008·Lab on a Chip·Samuel K Sia, Larry J Kricka
May 22, 2009·Lab on a Chip·Beom Seok LeeChristopher Ko
Dec 17, 2009·Biotechnology Advances·Vijayalakshmi VelusamyCatherine Adley
Jun 1, 2010·Lab on a Chip·Robert GorkinYoon-Kyoung Cho
Mar 8, 2011·Trends in Biotechnology·Angelika NiemzDavid S Boyle
Jan 27, 2012·Analytical Chemistry·Jiwoon ParkYoon-Kyoung Cho
Jan 1, 2009·Sensors·Jürgen MairhoferPeter Ertl
Sep 13, 2012·Sensors·Chia-Chen ChangChii-Wann Lin
Jan 17, 2013·Analytical Chemistry·Hyundoo HwangYoon-Kyoung Cho
Mar 19, 2014·Analytical Chemistry·Tae-Hyeong KimYoon-Kyoung Cho

❮ Previous
Next ❯

Citations

Jun 10, 2017·Sensors·Tsung-Feng WuIan Lian
Dec 21, 2018·Critical Reviews in Food Science and Nutrition·Yuting ShangXiulan Sun
Nov 15, 2018·Micromachines·Nurhaslina Abd RahmanMarc Madou
Feb 6, 2016·Micromachines·Minghui TangHo-Pui Ho
Mar 3, 2018·Sensors·Moo-Jung Seo, Jae-Chern Yoo

❮ Previous
Next ❯

Datasets Mentioned

BETA
NC003197.1

Methods Mentioned

BETA
biosensors
chip
chips
PCR
electrophoresis
nucleic acid amplification

Software Mentioned

Micro Total Analysis System
AutoCAD
LAMP Primer Explorer

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.