A portable epigenetic switch for bistable gene expression in bacteria

Scientific Reports
David R OlivenzaJosep Casadesús

Abstract

We describe a portable epigenetic switch based on opvAB, a Salmonella enterica operon that undergoes bistable expression under DNA methylation control. A DNA fragment containing the opvAB promoter and the opvAB upstream regulatory region confers bistability to heterologous genes, yielding OFF and ON subpopulations. Bistable expression under opvAB control is reproducible in Escherichia coli, showing that the opvAB switch can be functional in a heterologous host. Subpopulations of different sizes can be produced at will using engineered opvAB variants. Controlled formation of antibiotic-resistant and antibiotic-susceptible subpopulations may allow use of the opvAB switch in the study of bacterial heteroresistance to antibiotics.

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Citations

May 5, 2020·Environmental Microbiology·David R OlivenzaMireille Ansaldi

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Methods Mentioned

BETA
biosensors
biosensor
Flow cytometry
PCR

Software Mentioned

CXP
FlowJo8

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