A portable laser photostimulation and imaging microscope.

Journal of Neural Engineering
Volodymyr NikolenkoRafael Yuste

Abstract

We describe a compact microscope that uses a spatial light modulator (SLM) to control the excitation laser light. The flexibility of SLMs, which can mimic virtually any optical transfer function, enables the experimenter to create, in software, arbitrary spatio-temporal light patterns, including focusing and beam scanning, simply by calculating the appropriate phase mask. Our prototype, a scan-less device with no moving parts, can be used for laser imaging or photostimulation, supplanting the need for an elaborate optical setup. As a proof of principle, we generate complex excitation patterns on fluorescent samples and also perform functional imaging of neuronal activity in living brain slices.

References

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Jan 23, 2009·Nature Methods·Christoph LutzValentina Emiliani

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Citations

Nov 13, 2012·Nature Methods·Adam M PackerRafael Yuste
Jun 27, 2013·Nature Neuroscience·Adam M PackerMichael Häusser
Oct 22, 2010·Photomedicine and Laser Surgery
Mar 3, 2012·The Review of Scientific Instruments·Yafeng LiuShaoqun Zeng
Jan 3, 2012·Current Opinion in Neurobiology·Alipasha Vaziri, Valentina Emiliani
Jul 21, 2010·Journal of Neural Engineering·Shy Shoham, Karl Deisseroth
Apr 17, 2018·Current Opinion in Neurobiology·Weijian Yang, Rafael Yuste
Feb 23, 2018··Anna M. Linnenberger, Anna M. Linnenberger

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