A positron emission tomography image-guidable unimolecular micelle nanoplatform for cancer theranostic applications

Acta Biomaterialia
Jia YangWenjin Xu

Abstract

Unimolecular micelles based on hyperbranched polyamidoamine (PAMAM) dendrimer were synthesized as both a cargo delivery vector and an imaging agent for triple-negative breast tumors, and the chemical synthesis procedures are detailed in this study. With the chemical conjugation of a peptide (F3, against cellular nucleolin) to increase its cellular internalization, these micelles can accumulate potently and specifically in breast cancer cells (e.g., MDA-MB-231). The size and morphology of these PAMAM-based micelles have been measured by transmission electron microscopy (TEM) and dynamic light scattering (DLS). The hydrazone bond (responsive to pH alteration) between the loaded doxorubicin (DOX, as a model drug here) and PAMAM micelles enables cargo release following pH changes. Flow cytometry and confocal fluorescence microscopy revealed that PAMAM micelles with F3 attachment (PAMAM-DOX-F3) had stronger internalization into MDA-MB-231 cells (nucleolin-positive) than PAMAM micelles without F3 conjugation (PAMAM-DOX), whereas both of them have minimal interactions with L929 fibroblasts (nucleolin-negative). The positron-emitting isotope 64Cu was added into PAMAM micelles by chelation to track their pharmacokinetic behavior (organ ...Continue Reading

Citations

May 10, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Guangping YuShengsong Tang
Dec 13, 2019·Macromolecular Bioscience·Tingting XiaoMingwu Shen
Sep 20, 2020·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Haonan LiKui Luo
Oct 16, 2019·Mini Reviews in Medicinal Chemistry·Juliana de Oliveira Carneiro BrumJosé Daniel Figueroa Villar
May 16, 2020·Frontiers in Bioengineering and Biotechnology·Tingting LiYiyao Liu
Jan 16, 2021·Chemical Reviews·Mukaddes IzciStefaan J Soenen
Dec 25, 2019·Seminars in Cancer Biology·Muhammad AfzalFiroz Anwar

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