PMID: 699048Sep 1, 1978Paper

A possible mammary stem cell line

Cell
D C BennettP S Rudland

Abstract

The cell line Rama 25 is derived from a mammary tumor induced in a rat by dimethylbenzanthracene. During rapid proliferation, Rama 25 cells appear as a single undifferentiated epithelial type; at high cell densities, however, small numbers of two other cell types are formed, which respectively resemble secretory and myoepithelial cells of the mammary gland, as judged by light and electron microscopy and immunofluorescent staining for casein (milk proteins). These additional cell types cannot be explained as contaminating cell populations since the cell line has been cloned several times; furthermore, the proportion of either can be increased by dimethylsulphoxide under different conditions. Specific epithelial features are seen by histological and ultrastructural examination of tumors formed by Rama 25 cells in immunodeficient mice. A line of the myoepithelial-like cells, Rama 29, isolated from a Rama 25 culture by cloning, is also described. We propose that the undifferentiated cell type is a form of mammary stem cell which can differentiate in culture.

References

Sep 1, 1975·The Journal of Cell Biology·F G MoserF H Ruddle
Dec 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·T R Russell, R Ho
Aug 1, 1977·The Journal of Cell Biology·B T SchroederM S Soloff
Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J T EmermanS Nandi
Feb 1, 1977·The Journal of Cell Biology·J P Kraehenbuhl
Mar 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·R C ReubenP A Marks
Jul 1, 1971·In Vitro·E Y Lasfargues, D H Moore
Feb 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·C FriendT Sato
Apr 1, 1969·Cancer·M TannenbaumA J Marx
Nov 28, 1967·Biochimica Et Biophysica Acta·R W TurkingtonY J Topper
Jan 1, 1958·International Archives of Allergy and Applied Immunology·P G GELL
Mar 1, 1961·Experimental Cell Research·K E EBNERB L LARSON
Oct 1, 1964·The Journal of Cell Biology·E R WEIBEL, G E PALADE

❮ Previous
Next ❯

Citations

Jan 1, 1979·Journal of Supramolecular Structure·J E Lever
Jan 1, 1983·Cancer Metastasis Reviews·G H Heppner, B E Miller
Jan 1, 1981·Journal of Cancer Research and Clinical Oncology·F HölzelD Hanke
Jan 1, 1982·Virchows Archiv. A, Pathological Anatomy and Histology·B A GustersonM G Ormerod
Jan 1, 1984·Virchows Archiv. A, Pathological Anatomy and Histopathology·C A SmithA M Neville
Jan 1, 1981·Breast Cancer Research and Treatment·I S FentimanJ Taylor-Papadimitriou
Dec 1, 1986·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·U K EhmannW D Peterson
Mar 1, 1985·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·E J Ormerod, P S Rudland
Jan 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·E J Ormerod, P S Rudland
Feb 1, 1991·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·P S RudlandA M Platt-Higgins
Sep 1, 1991·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·U K EhmannL F Fajardo
Aug 1, 1982·In Vitro·S Sato, T Takeuchi
May 1, 1992·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·E Hellmén
Aug 2, 2011·Journal of Mammary Gland Biology and Neoplasia·Irma H Russo, Jose Russo
Oct 15, 1981·Developmental Biology·D C BennettS M Okada
Mar 1, 1983·Developmental Biology·E J OrmerodP S Rudland
Dec 1, 1985·Journal of Steroid Biochemistry·I Nenci
Sep 1, 1993·Immunology Today·M A Ritter, R L Boyd
Aug 1, 1980·Molecular and Cellular Endocrinology·B D BurleighS Strickland
Jul 1, 1981·Molecular and Cellular Endocrinology·B TeyssotL M Houdebine
Oct 1, 1983·Molecular and Cellular Endocrinology·J E Errick, T Kano-Sueoka
Dec 1, 1988·Molecular and Cellular Endocrinology·G F EricksonR Dulbecco
Oct 1, 1990·Molecular and Cellular Endocrinology·R Malienou-NgassaL M Houdebine
Jan 1, 1984·Biochimica Et Biophysica Acta·H S SmithA J Hackett
Aug 1, 1997·Biophysical Journal·Y E KorchevM J Lab
Dec 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·V A LennonR Dulbecco
Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R DulbeccoM Unger
Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J E Lever

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Cell eTOC

Cell is a scientific journal publishing research across a broad range of disciplines within the life sciences field. Discover the latest research from Cell here.