A Possible Mechanism: Genistein Improves Metabolism and Induces White Fat Browning Through Modulating Hypothalamic Expression of Ucn3, Depp , and Stc1

Frontiers in Endocrinology
Liyuan ZhouMingqun Deng

Abstract

Bioactive food components have gained growing attention in recent years. Multiple studies demonstrated that genistein had beneficial effects on metabolism. However, the exact mechanism by which genistein improves metabolism remains unclear, especially the central regulation. This study was designed to evaluate whether addition of genistein to the high-fat diet could counter metabolic disorders and whether these alterations were associated with gene expression in hypothalamus. C57BL/6 mice were fed either a high-fat diet (HF), high-fat diet with genistein (0.25 g/kg diet) (HFG) or a normal control diet (CON) for 8 weeks. Body weight was assessed during the study. After 8-week intervention, content of inguinal subcutaneous adipose tissue (SAT), perirenal visceral adipose tissue (VAT) and brown adipose tissue (BAT) were weighed. Glucose tolerance test, the serum levels of insulin and lipid were assessed. The mRNA of browning marker was detected in the white fat. The hypothalamus was collected for whole transcriptome sequencing and reverse transcription quantitative PCR validation. The results demonstrated that mice fed HFG diet had lower body weight and SAT mass, decrease levels of low-density lipoprotein cholesterol and free fatt...Continue Reading

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Citations

Apr 16, 2020·The Proceedings of the Nutrition Society·Carla Horvath, Christian Wolfrum
Dec 5, 2019·International Journal of Molecular Sciences·Silvia Lorente-CebriánHeriberto Castro
Oct 9, 2020·Molecular and Cellular Biochemistry·Suresh C TyagiMahavir Singh
Jan 1, 2021·International Journal of Molecular Sciences·Alina Kuryłowicz
Mar 13, 2021·Frontiers in Physiology·Mahavir SinghSuresh C Tyagi
Mar 16, 2021·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Dong-Min HouWan-Long Zhu

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Datasets Mentioned

BETA
SRP187329

Methods Mentioned

BETA
ELISA
RNA Assay
PCR

Software Mentioned

cBot Cluster Generation System
Tophat2
GraphPad
DESeq R
KOBASsoftware
DESeq
Prism
Volcano Plot

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