A possible mechanism underlying corymine inhibition of glycine-induced Cl- current in Xenopus oocytes

European Journal of Pharmacology
P LeewanichH Watanabe

Abstract

We previously reported that corymine, an alkaloid extracted from the leaves of Hunteria zeylanica native to Thailand, inhibited glycine-induced chloride current using a receptor expression model of Xenopus oocytes. In this study, we investigated the mechanism underlying the inhibitory action of this alkaloid on glycine current using the same model. Corymine inhibited glycine current in a noncompetitive fashion. Co-application with strychnine, a competitive glycine receptor antagonist, or 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS), a Cl- channel blocker, corymine decreased the ED50 value of strychnine, but did not change that of DIDS. Moreover, the inhibitory effects of corymine and either strychnine or DIDS were additive. The desensitization phase of glycine current showed two exponentials and corymine preferentially inhibited the fast component, whereas strychnine affected both of them to the same extent and DIDS preferentially inhibited the slow component. When these drugs were applied repeatedly, the inhibitory effects of corymine and strychnine were not use-dependent and reversible, while the effect of DIDS was use-dependent and irreversible. The inhibitory effect of corymine on gamma-aminobutyric acid (GABA) c...Continue Reading

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Citations

Nov 16, 2002·European Journal of Pharmacology·Tai-Hyun KangHiroshi Watanabe
Aug 5, 2003·Pharmacology, Biochemistry, and Behavior·Hiroshi WatanabeHiromitsu Takayama
Sep 19, 2012·The Journal of Physiology·Portia A KunzBenjamin D Philpot
May 13, 2005·Journal of Pharmacological Sciences·Pathama LeewanichKinzo Matsumoto
Dec 31, 2005·Journal of Neurophysiology·Weifeng SongLori L McMahon
May 18, 2010·The Journal of Organic Chemistry·Yusuke HirasawaHiroshi Morita
Apr 25, 2009·Journal of Natural Products·Alfarius E NugrohoHiroshi Morita

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