A possible role for iron-sulfur cluster N2 in proton translocation by the NADH: ubiquinone oxidoreductase (complex I)

Journal of Molecular Microbiology and Biotechnology
Dirk FlemmingThorsten Friedrich

Abstract

The proton-pumping NADH:ubiquinone oxidoreductase, the respiratory complex I, couples the transfer of electrons from NADH to ubiquinone with the translocation of protons across the membrane. The enzyme mechanism is still unknown due to the lack of a high-resolution structure and its complicated composition. The complex from Escherichia coli is made up of 13 subunits called NuoA through NuoN and contains one FMN and nine iron-sulfur (Fe/S) clusters as redox groups. The pH dependence of the midpoint redox potential of the Fe/S cluster named N2 and its spin-spin interaction with ubiquinone radicals made it an ideal candidate for a key component in redox-driven proton translocation. During the past years we have assigned the subunit localization of cluster N2 to subunit NuoB by site-directed mutagenesis and predicted its ligation by molecular simulation. Redox-induced FT-IR spectroscopy has shown that its redox reaction is accompanied by the protonation and deprotonation of individual amino acid residues. These residues have been identified by site-directed mutagenesis. The enzyme catalytic activity depends on the presence of cluster N2 and is coupled with major conformational changes. From these data a model for redox-induced conf...Continue Reading

References

Aug 1, 1992·Quarterly Reviews of Biophysics·J E Walker
May 8, 1991·European Journal of Biochemistry·H WeissD Preis
Feb 4, 1974·Biochemical and Biophysical Research Communications·T OnishiE Racker
Apr 1, 1995·Journal of Bacteriology·R L KerbyG P Roberts
Feb 16, 1995·Nature·A VolbedaJ C Fontecilla-Camps
Aug 1, 1993·Journal of Bioenergetics and Biomembranes·T FriedrichH Weiss
Aug 1, 1993·Journal of Bioenergetics and Biomembranes·V D SledT Ohnishi
Sep 13, 1993·Biochimica Et Biophysica Acta·I E Hassinen, P T Vuokila
Jan 16, 1997·Biochimica Et Biophysica Acta·S P AlbrachtP de Jong
Apr 7, 1997·Biochemical and Biophysical Research Communications·K Young-MogT Tsuchiya
Aug 21, 1997·Journal of Theoretical Biology·T Friedrich, H Weiss
Jun 19, 1998·Biochimica Et Biophysica Acta·T Friedrich
Jun 19, 1998·Biochimica Et Biophysica Acta·A DupuisJ P Issartel
Jun 19, 1998·Biochimica Et Biophysica Acta·T Ohnishi
Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·F SchulerJ E Casida
Aug 17, 1999·Current Opinion in Structural Biology·N Grigorieff
Sep 22, 1999·European Journal of Biochemistry·J MeuerR Hedderich

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Citations

Jan 17, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Wenyue SunJoseph A Califano
Mar 17, 2010·Biochimica Et Biophysica Acta·Stéphane RansacJean-Pierre Mazat
Mar 6, 2012·Biochimica Et Biophysica Acta·Rouslan G Efremov, Leonid A Sazanov
Jan 19, 2016·Biochimica Et Biophysica Acta·Gerhard Hummer, Mårten Wikström
Dec 26, 2006·The Journal of Biological Chemistry·Aaron M GusdonClayton E Mathews
Apr 1, 2007·EcoSal Plus·Thorsten Friedrich, Thomas Pohl
May 25, 2010·Biochimica Et Biophysica Acta·Maja A TocilescuUlrich Brandt

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