A possible role for potassium and pyruvate in the modulation of sperm motility in the rabbit oviducal isthmus

Journal of Reproduction and Fertility
L J BurkmanD F Katz

Abstract

Spermatozoa were recovered from the isthmus of the rabbit oviduct at 4 and 11 h post coitum using several defined flushing media. The motility of spermatozoa in the isthmic flushings was subsequently analysed from video recordings. There was little sperm movement in the native isthmic fluid, but vigorous flagellar activity and hyperactivated movement were induced by flushing the isthmus with 0.25 M-sucrose, apparently an effect of dilution. Flushing with a complex culture medium resulted in similar stimulation of sperm movement. When pyruvate was present in the medium, hyperactivated flagellar bending was stimulated, whereas these movements were virtually absent when glucose alone was present. The stimulating effect of dilution was less pronounced when the flushing medium contained 50 mM-potassium. When the isthmus was flushed with media containing 50 mM-K+ and the K+ concentration was lowered to 5 mM during a washing procedure, large-amplitude flagellar movements were sequentially suppressed and restored. The restoration of large-amplitude movements was enhanced when pyruvate was present in the 5 mM-K+ washing medium. These results suggest that alterations in the concentration of both K+ and pyruvate may have a role in regulat...Continue Reading

Citations

Dec 31, 2002·Chemistry and Physics of Lipids·Herbert SchuelAndrea Giuffrida
May 23, 2009·Systems Biology in Reproductive Medicine·Jian-Yuan JinQi-Xian Shi
Dec 5, 2008·Systems Biology in Reproductive Medicine·Marcelo MarconiFernando Romero
Oct 24, 2006·Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine·E AkcayT Katila
Dec 17, 2008·Reproduction in Domestic Animals = Zuchthygiene·D RathU Taylor
Dec 1, 1991·International Journal of Andrology·C L Barratt, I D Cooke
Mar 1, 1990·Anatomia, histologia, embryologia·H Rodriguez-MartinezK Larsson
Sep 19, 2002·Molecular Reproduction and Development·Herbert SchuelAlexandros Makriyannis
Mar 1, 1988·Journal of Andrology·L J Burkman
Jun 11, 2008·Journal of Proteome Research·Adil SeytanogluAlireza Fazeli

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