A postnatal peak in microglial development in the mouse hippocampus is correlated with heightened sensitivity to seizure triggers

Brain and Behavior
Iris KimS Koh

Abstract

Explosive synaptogenesis and synaptic pruning occur in the hippocampus during the first two weeks of postnatal life, coincident with a heightened susceptibility to seizures in rodents. To determine the temporal correlation between microglial development and age-dependent susceptibility and response to seizures, we quantified developmental changes in basal microglia levels and seizure-induced microglial activation in the hippocampus of Cx3Cr1(GFP /+) transgenic mice. Basal levels of microglia were quantified in the hippocampi of Cx3Cr1(GFP /+) mice at P0, P5, P10, P15, P20, P25, P30, P40, and P60. Seizure susceptibility and seizure-induced microglial activation were assessed in response to febrile seizures (lipopolysaccharide followed by hyperthermia) and kainic acid-induced status epilepticus. The density of microglia within the hippocampus increased rapidly after birth, reaching a peak during the second week of life - the age at which the animals became most vulnerable to seizure triggers. In addition, this peak of microglial development and seizure vulnerability during the second postnatal week represented the time of maximal seizure-induced microglia activation. Overreactive innate immunity mediated by activated microglia ma...Continue Reading

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Citations

Jun 19, 2016·Journal of Neuroinflammation·Franck VerdonkAnne Danckaert
Sep 2, 2016·Epilepsy Currents·Carl E Stafstrom, Eric M Kossoff
Oct 30, 2016·Neuroscience and Biobehavioral Reviews·Victoria A Macht
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Nov 13, 2021·Proceedings of the National Academy of Sciences of the United States of America·Pablo J LitumaSayan Nandi

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Methods Mentioned

BETA
PCR
fluorescence microscopy
flow cytometry
FACS

Key Resources (RRID) Mentioned

nif
AB_2224402
AB_1500720
AB_394657
AB_1732076
AB_830649
AB_11203704
AB_10697046
AB_312673
AB_1877271

Software Mentioned

GraphPad
Image J64
ImageJ
FlowJo

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