Abstract
Kaposi's sarcoma-associated herpesvirus (KSHV; also known as human herpesvirus 8, HHV-8) belongs to the gamma-herpesvirus subfamily. The KSHV ORF57 gene is thought to be a homolog of posttranscriptional regulators that are conserved in the herpesvirus family and are essential for replication. We generated specific monoclonal antibodies (mAbs) against the ORF57 protein that detected the 51-kDa protein expressed in the nucleus of KSHV-infected cells. We also found that the ORF57 protein interacted with poly(rC)-binding protein 1 (PCBP1), a cellular RNA-binding, posttranscriptional regulator. ORF57's interaction with PCBP1 enhanced the activity of not only poliovirus internal ribosome-entry site (IRES)-dependent translation but also X-linked inhibitor of apoptosis (XIAP) and KSHV vFLIP IRES. Actually, when ORF57 expression was induced by the expression of replication and transcription activator (RTA) in KSHV-infected cells, the expression of XIAP was enhanced. These results suggest that ORF57 binds to PCBP1 as a functional partner for posttranscriptional regulation and is involved in the regulation of the expression of both cellular and viral genes through IRESs.
References
Mar 23, 1978·Nature·H zur HausenE Hecker
Jun 1, 1990·Virology·T OkunoK Yamanishi
Jun 1, 1990·The Journal of General Virology·S EfstathiouU A Gompels
Dec 16, 1994·Science·Y ChangP S Moore
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A PhelanJ B Clements
May 16, 1997·Cell·D H OstareckM W Hentze
Jun 13, 1997·Cell·A B SachsM W Hentze
Jun 1, 1997·The EMBO Journal·W M MichaelG Dreyfuss
Dec 16, 1998·Trends in Biochemical Sciences·A Ostareck-LedererM W Hentze
Feb 6, 1999·The Journal of General Virology·Y YuM K Offermann
Oct 3, 1999·The Journal of Biological Chemistry·S WaddJ B Clements
Jan 7, 2000·Journal of Virology·A K GuptaS Swaminathan
Mar 25, 2000·Molecular and Cellular Biology·A OumardM Nourbakhsh
Jun 10, 2000·Molecular and Cellular Biology·M Holcik, R G Korneluk
Oct 26, 2000·Trends in Genetics : TIG·M HolcikR G Korneluk
Apr 21, 2001·Molecular and Cellular Biology·S A MitchellA E Willis
Jan 29, 2002·Nucleic Acids Research·Y K KimS K Jang
May 11, 2002·RNA·Aleksandr V Makeyev, Stephen A Liebhaber
Dec 17, 2002·Molecular and Cellular Biology·Martin HolcíkRobert G Korneluk
Jan 16, 2003·Nucleic Acids Research·Becky M PickeringAnne E Willis
Nov 6, 2003·Virology·Ken NishimuraKoichi Yamanishi
Citations
Oct 5, 2007·Journal of Virology·Diana PalmeriDavid M Lukac
Dec 14, 2007·Journal of Virology·Gaby SanderMichael Stürzl
May 16, 2008·Journal of Virology·John P BilelloRonald C Desrosiers
Mar 11, 2009·Frontiers in Bioscience (Landmark Edition)·Vladimir Majerciak, Zhi-Ming Zheng
Oct 27, 2011·PLoS Pathogens·Shaoying LeeRen Sun
Feb 13, 2015·Viruses·Vladimir Majerciak, Zhi-Ming Zheng
Feb 14, 2012·Leukemia Research·Sampath RamachandiranLeon Bernal-Mizrachi
Mar 12, 2010·PLoS Pathogens·Brooke B SahinNicholas K Conrad
Aug 5, 2017·Pathogens·Jennifer L DeCotiis, David M Lukac
Feb 20, 2015·Journal of Virology·Carolin VogtJens Bohne
Nov 8, 2013·Journal of Virology·Erik M LenarcicNathaniel J Moorman
Feb 4, 2014·PloS One·Zhen LuoJianguo Wu
Aug 21, 2019·Virologica Sinica·Yan ZhangChangchun Tu
Oct 21, 2006·Biochemical Society Transactions·P Malik, E C Schirmer
Jul 11, 2006·The Journal of Biological Chemistry·Vladimir MajerciakZhi-Ming Zheng
Nov 6, 2018·Viruses·Mara Cirone
Aug 10, 2017·Pathogens·Jennifer L DeCotiis, David M Lukac
Oct 16, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Xuetian ZhangHong Zhang
Feb 26, 2021·Journal of Virology·Atsuko SugimotoMasahiro Fujimuro
Nov 18, 2021·Journal of Virology·Beatriz Alvarado-HernandezZhi-Ming Zheng