A posttranscriptional regulator of Kaposi's sarcoma-associated herpesvirus interacts with RNA-binding protein PCBP1 and controls gene expression through the IRES

Virology
Ken NishimuraKoichi Yamanishi

Abstract

Kaposi's sarcoma-associated herpesvirus (KSHV; also known as human herpesvirus 8, HHV-8) belongs to the gamma-herpesvirus subfamily. The KSHV ORF57 gene is thought to be a homolog of posttranscriptional regulators that are conserved in the herpesvirus family and are essential for replication. We generated specific monoclonal antibodies (mAbs) against the ORF57 protein that detected the 51-kDa protein expressed in the nucleus of KSHV-infected cells. We also found that the ORF57 protein interacted with poly(rC)-binding protein 1 (PCBP1), a cellular RNA-binding, posttranscriptional regulator. ORF57's interaction with PCBP1 enhanced the activity of not only poliovirus internal ribosome-entry site (IRES)-dependent translation but also X-linked inhibitor of apoptosis (XIAP) and KSHV vFLIP IRES. Actually, when ORF57 expression was induced by the expression of replication and transcription activator (RTA) in KSHV-infected cells, the expression of XIAP was enhanced. These results suggest that ORF57 binds to PCBP1 as a functional partner for posttranscriptional regulation and is involved in the regulation of the expression of both cellular and viral genes through IRESs.

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Citations

Dec 14, 2007·Journal of Virology·Gaby SanderMichael Stürzl
Mar 11, 2009·Frontiers in Bioscience (Landmark Edition)·Vladimir Majerciak, Zhi-Ming Zheng
Feb 13, 2015·Viruses·Vladimir Majerciak, Zhi-Ming Zheng
Aug 5, 2017·Pathogens·Jennifer L DeCotiis, David M Lukac
Aug 21, 2019·Virologica Sinica·Yan ZhangChangchun Tu
Oct 21, 2006·Biochemical Society Transactions·P Malik, E C Schirmer
Aug 10, 2017·Pathogens·Jennifer L DeCotiis, David M Lukac
Oct 16, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Xuetian ZhangHong Zhang
Nov 18, 2021·Journal of Virology·Beatriz Alvarado-HernandezZhi-Ming Zheng

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